Hirayama Masahiro, Azuma Eiichi
Department of Pediatrics and Mie University Graduate School of Medicine; Tsu, Mie Japan.
Chimerism. 2011 Jan;2(1):23-4. doi: 10.4161/chim.2.1.15127.
The immunologic effects of developmental exposure to non-inherited maternal antigens (NIMA) are heterogeneous, either tolerogenic or immunogenic. The role of minor histocompatibility antigens (MiHA) in NIMA effects is unknown. We have recently reported that the NIMA effect can be classified into two distinct reactivities, low and high responder, to NIMA in utero and during nursing depending on the degree of maternal microchimerism (MMc) and Foxp3 expression of peripheral blood CD4(+)CD25(+) cells after graft-versus-host disease (GVHD) induction. These reactivities were predictable before transplantation, using an MLR-ELISPOT (mixed lymphocyte reaction; enzyme-linked immunospot) assay by comparing the number of IFNγ-producing cells stimulated with NIMA. Moreover, this assay was also applicable in both major and minor NIMA-mismatched setting. These observations are clinically relevant and suggest that it is possible to predict the immunological tolerance to NIMA.
发育过程中暴露于非遗传母体抗原（NIMA）的免疫效应是异质性的，既可以是致耐受性的，也可以是免疫原性的。次要组织相容性抗原（MiHA）在NIMA效应中的作用尚不清楚。我们最近报道，根据移植物抗宿主病（GVHD）诱导后母体微嵌合体（MMc）的程度以及外周血CD4(+)CD25(+)细胞的Foxp3表达，NIMA效应可分为对子宫内和哺乳期NIMA的两种不同反应性，即低反应者和高反应者。在移植前，通过使用MLR-ELISPOT（混合淋巴细胞反应；酶联免疫斑点）试验，比较用NIMA刺激产生IFNγ的细胞数量，这些反应性是可预测的。此外，该试验在主要和次要NIMA不匹配的情况下均适用。这些观察结果具有临床相关性，并表明有可能预测对NIMA的免疫耐受性。
