Bigelow Laboratory for Ocean Sciences, West Boothbay Harbor, ME 04575, USA.
Science. 2011 May 6;332(6030):714-7. doi: 10.1126/science.1203163.
Whole-genome shotgun sequence data from three individual cells isolated from seawater, followed by analysis of ribosomal DNA, indicated that the cells represented three divergent clades of picobiliphytes. In contrast with the recent description of this phylum, we found no evidence of plastid DNA nor of nuclear-encoded plastid-targeted proteins, which suggests that these picobiliphytes are heterotrophs. Genome data from one cell were dominated by sequences from a widespread single-stranded DNA virus. This virus was absent from the other two cells, both of which contained non-eukaryote DNA derived from marine Bacteroidetes and large DNA viruses. By using shotgun sequencing of uncultured marine picobiliphytes, we revealed the distinct interactions of individual cells.
从海水中分离出的三个单细胞的全基因组鸟枪法测序数据，结合核糖体 DNA 分析，表明这些细胞代表了三个不同的束毛藻门分支。与最近对该门的描述不同，我们没有发现质体 DNA 或核编码的质体靶向蛋白的证据，这表明这些束毛藻是异养生物。一个细胞的基因组数据主要由一种广泛存在的单链 DNA 病毒的序列组成。这种病毒不存在于另外两个细胞中，这两个细胞都含有来自海洋拟杆菌门和大型 DNA 病毒的非真核 DNA。通过对未培养的海洋束毛藻的鸟枪法测序，我们揭示了单个细胞的独特相互作用。
