Weng Huachun, Shen Chunshen, Hirokawa Gou, Ji Xu, Takahashi Rie, Shimada Kana, Kishimoto Chiharu, Iwai Naoharu
Department of Genomic Medicine, Research Institute, National Cerebral and Cardiovascular Center, Suita, Osaka, Japan.
Biomed Res. 2011 Apr;32(2):135-41. doi: 10.2220/biomedres.32.135.
MicroRNAs (miRNAs) are endogenous small RNAs that play an important role in various physiological processes by downregulating target genes. Recently, plasma miRNAs have been investigated as biomarkers for various diseases. In this study, miRNA array analysis in various tissues showed that miR-124 is almost exclusively expressed in the central nervous system and neuronal cells, suggesting that it might be useful as a potential biomarker for neurological diseases. We examined whether plasma concentrations of brain-specific miRNA can serve as a biomarker for cerebral infarction, where the cerebral infarction was modeled by middle cerebral artery occlusion (MCAO) in the rat. Plasma concentrations of miR-124 were significantly elevated at 6 h, and remained elevated at 48 h after MCAO introduction. Thus, plasma concentration of miR-124 provides a promising candidate biomarker for early detection of cerebral infarction.
微小RNA（miRNA）是内源性小RNA，通过下调靶基因在各种生理过程中发挥重要作用。最近，血浆miRNA已被研究作为各种疾病的生物标志物。在本研究中，对各种组织进行的miRNA阵列分析表明，miR-124几乎仅在中枢神经系统和神经元细胞中表达，这表明它可能作为神经疾病的潜在生物标志物。我们研究了脑特异性miRNA的血浆浓度是否可作为脑梗死的生物标志物，其中通过大鼠大脑中动脉闭塞（MCAO）建立脑梗死模型。miR-124的血浆浓度在6小时时显著升高，并在MCAO引入后48小时保持升高。因此，miR-124的血浆浓度为脑梗死的早期检测提供了一个有前景的候选生物标志物。
