Department of Biology, Lund University, Sölvegatan 35, 223 62 Lund, Sweden.
Nat Commun. 2011;2:302. doi: 10.1038/ncomms1305.
Saccharomyces yeasts degrade sugars to two-carbon components, in particular ethanol, even in the presence of excess oxygen. This characteristic is called the Crabtree effect and is the background for the 'make-accumulate-consume' life strategy, which in natural habitats helps Saccharomyces yeasts to out-compete other microorganisms. A global promoter rewiring in the Saccharomyces cerevisiae lineage, which occurred around 100 mya, was one of the main molecular events providing the background for evolution of this strategy. Here we show that the Dekkera bruxellensis lineage, which separated from the Saccharomyces yeasts more than 200 mya, also efficiently makes, accumulates and consumes ethanol and acetic acid. Analysis of promoter sequences indicates that both lineages independently underwent a massive loss of a specific cis-regulatory element from dozens of genes associated with respiration, and we show that also in D. bruxellensis this promoter rewiring contributes to the observed Crabtree effect.
酿酒酵母将糖降解为二碳成分，特别是乙醇，即使在有过量氧气的情况下也是如此。这种特性称为克雷布斯效应，是“制造-积累-消耗”生活策略的背景，在自然栖息地中，该策略有助于酿酒酵母胜过其他微生物。大约 1 亿年前，酿酒酵母谱系中的一个全局启动子重布线是为该策略的进化提供背景的主要分子事件之一。在这里，我们表明，与酿酒酵母分离超过 2 亿年前的德克氏酵母谱系也能有效地制造、积累和消耗乙醇和乙酸。启动子序列分析表明，这两个谱系都独立地从数十个与呼吸有关的基因中丢失了一个特定的顺式调控元件，我们还表明，在德克氏酵母中，这种启动子重布线也有助于观察到的克雷布斯效应。
