Gao X, Zacharek A, Grignon D, Liu H, Sakr W, Porter A, Chen Y, Honn K
WAYNE STATE UNIV,SCH MED,DEPT PATHOL,DETROIT,MI 48201. WAYNE STATE UNIV,DEPT CHEM,DETROIT,MI 48201. WAYNE STATE UNIV,SCH MED,DEPT RADIAT ONCOL,DIV CANC BIOL,DETROIT,MI 48201.
Int J Oncol. 1995 Jan;6(1):111-7. doi: 10.3892/ijo.6.1.111.
Loss of heterozygosity (LOH) or allelic deletion at various loci has been reported in the majority of human tumors. The frequently deleted targets are believed to be tumor suppressor genes. Recent studies have identified the APC and MCC genes at 5q21, as putative tumor suppressor genes. The APC and MCC genes have been implicated in the development of familial adenomatous polyposis coli and cancers of the gastrointestinal tract, ovary, breast and lung. In the present study, we investigated a possible role of the APC and MCC genes in prostate cancer development. mRNA expression of the APC and MCC genes and LOH at the APC and MCC loci were determined in prostate cancer tissues from 28 patients and 5 human prostatic adenocarcinoma cell lines. Of the informative cases, the frequency of LOH at the APC and MCC loci was 63% (10/16) and 54% (7/13), respectively. Overall, 65% (15/23) of the informative cases showed LOH at the APC and/or MCC gene. All prostate cancer cell lines showed homozygosity at all APC and MCC polymorphic sites studied. Approximately half (57%) of the tumor tissues examined showed a decreased expression of APC and MCC mRNA. Our data suggest that the APC and MCC genes may be involved in the formation of human prostate cancer (HPC).
在大多数人类肿瘤中都报道了不同位点的杂合性缺失（LOH）或等位基因缺失。频繁缺失的靶点被认为是肿瘤抑制基因。最近的研究已确定位于5q21的APC和MCC基因为假定的肿瘤抑制基因。APC和MCC基因与家族性腺瘤性息肉病以及胃肠道、卵巢、乳腺和肺癌有关。在本研究中，我们调查了APC和MCC基因在前列腺癌发生中的可能作用。测定了28例患者的前列腺癌组织和5个人前列腺腺癌细胞系中APC和MCC基因的mRNA表达以及APC和MCC位点的LOH。在信息充分的病例中，APC和MCC位点的LOH频率分别为63%（10/16）和54%（7/13）。总体而言，65%（15/23）的信息充分病例在APC和/或MCC基因处显示LOH。所有前列腺癌细胞系在所研究的所有APC和MCC多态性位点均显示纯合性。大约一半（57%）的检测肿瘤组织显示APC和MCC mRNA表达降低。我们的数据表明，APC和MCC基因可能参与了人类前列腺癌（HPC）的形成。
