Advanced Research Center, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Chuo-ku, Niigata, 951-8580, Japan.
Odontology. 2012 Jan;100(1):10-21. doi: 10.1007/s10266-011-0020-7. Epub 2011 May 10.
In an effort to identify a possible role for type III collagen in the morphogenesis of circumvallate papillae on the surface of the rat tongue, we examined its appearance by fluorescent immunostaining, in conjunction with differential interference contrast images and images obtained, after staining with toluidine blue, in the transmission mode by laser-scanning microscopy. We analyzed semi-ultrathin sections of epoxy resin-embedded samples of the lingual mucosa of embryonic and juvenile rats, 13 days after conception (E13) to day 21 after birth (P21). Immunoreactivity specific for type III collagen was recognized first in the mesenchymal connective tissue just beneath the circumvallate papilla placode in fetuses on E13. At this stage, most of the lingual epithelium with the exception of the circumvallate papilla placode was pseudostratified epithelium composed of one or two layers of cuboidal cells. However, the epithelium of the circumvallate papilla placode was composed of several layers of cuboidal cells. Immunoreactivity specific for type III collagen was detected mainly on the lamina propria just beneath the lingual epithelium of the rudiment of the circumvallate papilla and the developing circumvallate papilla in fetuses on E15 and E17, and slight immunostaining was detected on the lamina propria around the rudiment. In fetuses on E19, immunoreactivity specific for type III collagen was widely and densely distributed on the connective tissue around the developing circumvallate papillae and, also, on the connective tissue that surrounded the lingual muscle. However, the immunoreactivity specific for type III collagen was sparsely distributed on the lamina propria of each central papillar structure. After birth, from P0 to P14, morphogenesis of the circumvallate papillae advanced gradually with the increase in the total volume of the tongue. At these postnatal stages, the intensity of the fluorescence due to immunoreactivity specific for type III collagen was distinctively distributed on the lamina propria around each circumvallate papilla, on each central bulge and on the connective tissue that surrounded the lingual muscle. However, immunofluorescence was less distinct on the connective tissue that surrounded the lingual muscle. Thus, type III collagen appeared in conjunction with the morphogenesis of the circumvallate papillae, as well as in the connective tissue that surrounded the lingual muscle during myogenesis of the rat tongue.
为了确定 III 型胶原在大鼠舌表面嵴状乳头形态发生中的可能作用，我们通过荧光免疫染色，结合微分干涉对比图像和甲苯胺蓝染色后的透射模式激光扫描显微镜图像，对其进行了研究。我们分析了胚胎和幼鼠舌黏膜的半超薄环氧树脂嵌入样本，这些样本是在受孕后 13 天（E13）到出生后 21 天（P21）获得的。在 E13 日的胎儿中，III 型胶原的免疫反应性首先在嵴状乳头嵴状乳头帽状下的间质结缔组织中被识别。在这个阶段，除了嵴状乳头帽状下的舌上皮外，大部分舌上皮是由一层或两层立方细胞组成的假复层上皮。然而，嵴状乳头帽状下的上皮由数层立方细胞组成。在 E15 和 E17 日的胎儿中，III 型胶原的免疫反应性主要在嵴状乳头和发育中的嵴状乳头的原始舌上皮下的固有层中被检测到，在原始周围的固有层中也检测到轻微的免疫染色。在 E19 日的胎儿中，III 型胶原的免疫反应性广泛而密集地分布在发育中的嵴状乳头周围的结缔组织中，也分布在环绕舌肌的结缔组织中。然而，III 型胶原的免疫反应性在每个中央乳突结构的固有层中稀疏分布。出生后，从 P0 到 P14，随着舌总容积的增加，嵴状乳头的形态发生逐渐进展。在这些出生后的阶段，III 型胶原免疫反应性的荧光强度在每个嵴状乳头周围的固有层、每个中央隆突和环绕舌肌的结缔组织上明显分布。然而，环绕舌肌的结缔组织的免疫荧光不那么明显。因此，III 型胶原在大鼠舌的肌发生过程中，与嵴状乳头的形态发生以及环绕舌肌的结缔组织一起出现。
