Löchelt M, Haas L, Kaaden O R
Institute for Virology, Hannover Veterinary School, F.R.G.
J Gen Virol. 1990 Mar;71 ( Pt 3):541-9. doi: 10.1099/0022-1317-71-3-541.
In this report we present data indicating that the recently detected monomer covalently closed linear replicative form DNA (Mccl RF DNA) is an intermediate of Aleutian disease virus (ADV) DNA replication. This DNA molecule is characterized by covalently closed terminal hairpins on either end of the linear ds genomic molecule. Its first detection early after infection in vitro, the association with ADV-specific replication complexes and the de novo synthesis of Mccl RF DNA by isolated replication complexes point to an important role in parvoviral DNA replication. The presence of the Mccl RF DNA in extracts of virus-producing bone marrow cells of naturally infected mink rules out the possibility that this DNA form represents a mere cell culture artefact. The detection of the ADV non-structural protein 1 (NS1) in replication complexes supports the view that this protein is involved in ADV DNA replication.
在本报告中，我们展示的数据表明，最近检测到的单体共价闭合线性复制型DNA（Mccl RF DNA）是阿留申病病毒（ADV）DNA复制的中间体。该DNA分子的特征是线性双链基因组分子两端存在共价闭合的末端发夹结构。它在体外感染后早期首次被检测到，与ADV特异性复制复合物相关联，并且分离出的复制复合物能从头合成Mccl RF DNA，这表明其在细小病毒DNA复制中起重要作用。在自然感染水貂的病毒产生骨髓细胞提取物中存在Mccl RF DNA，排除了这种DNA形式仅仅是细胞培养假象的可能性。在复制复合物中检测到ADV非结构蛋白1（NS1），支持了这种蛋白质参与ADV DNA复制的观点。
