Center for Radiological Research, College of Physicians & Surgeons, Columbia University, New York, NY, USA.
Cancer Lett. 2011 Sep 1;308(1):23-32. doi: 10.1016/j.canlet.2011.04.010. Epub 2011 May 10.
Controversy has arisen as to the role of transforming growth factor-β-induced protein (TGFBI) in the regulation of tumor metastasis. Using lung and breast cancer cell lines (H522 and MCF-7, respectively), we established that TGFBI induced cell adhesion to extracellular matrix proteins by activating adhesion-associated signaling and subsequent structure reformation, ultimately leading to cells less motile; whereas TGFBI reduced abilities of colony formation in soft agar, penetration through matrix gel, and activation of matrix metalloproteinases 2 and 9. Furthermore, injection of TGFBI-expressing cells into immuno-deficient mice resulted in a significant reduction in tumor metastasis in vivo. Taken together, these data suggest that TGFBI moderates the metastatic potential of cancer cells.
转化生长因子-β诱导蛋白（TGFBI）在肿瘤转移调控中的作用存在争议。本研究使用肺癌和乳腺癌细胞系（H522 和 MCF-7），发现 TGFBI 通过激活黏附相关信号转导和随后的结构重塑，诱导细胞黏附到细胞外基质蛋白，最终导致细胞迁移能力降低；而 TGFBI 降低了细胞在软琼脂中的集落形成能力、穿过基质胶的穿透能力以及基质金属蛋白酶 2 和 9 的激活能力。此外，将表达 TGFBI 的细胞注射到免疫缺陷小鼠体内，可显著降低体内肿瘤转移。综上所述，这些数据表明 TGFBI 调节癌细胞的转移潜能。
