开发和鉴定巴西栗树 Bertholletia excelsa Humb. & Bonpl. (Lecythidaceae) 的微卫星标记。

Development and characterization of microsatellite markers for the Brazil nut tree Bertholletia excelsa Humb. & Bonpl. (Lecythidaceae).

机构信息

Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, SGAN 916, Mod. B, Asa Norte, Brasília, DF 70790-160, Brazil Laboratório de Genética e Biologia Reprodutiva de Plantas, Instituto Nacional de Pesquisas na Amazônia (INPA), Av. André Araújo, 2936, Manaus, AM 69083-000, Brazil.

出版信息

Mol Ecol Resour. 2009 May;9(3):920-3. doi: 10.1111/j.1755-0998.2008.02481.x. Epub 2009 Jan 22.

DOI:10.1111/j.1755-0998.2008.02481.x

PMID:21564791

Abstract

Twelve polymorphic microsatellite markers were developed for the Brazil nut (Bertholletia excelsa), one of the most valuable non-timber forest products from the Amazon, based on enrichment protocol. Six to 18 (mean 10.4) alleles per locus were identified and the expected heterozygosity ranged from 0.663 to 0.923 based on a screen of 40 individuals from one population of B. excelsa. The combined probabilities of genetic identity (8.39 × 10(-17) ) and paternity exclusion (0.999999) indicated that multilocus genotypes are likely to be unique allowing precise analyses of genetic structure, gene flow, and mating system of this economically important species.

摘要

基于富集方案，为巴西坚果（Bertholletia excelsa），即亚马逊地区最有价值的非木材林产品之一，开发了 12 个多态性微卫星标记。在对来自巴西坚果单一群体的 40 个个体进行筛查后，每个位点可识别 6 到 18 个（平均 10.4 个）等位基因，预期杂合度范围为 0.663 到 0.923。遗传同一性（8.39×10（-17））和父系排除（0.999999）的综合概率表明，多位点基因型可能是独特的，从而能够对该具有重要经济意义的物种的遗传结构、基因流和交配系统进行精确分析。