Leung H, Hughes C, Kloppel G, Williamson R, Lemoine N
HAMMERSMITH HOSP,ROYAL POSTGRAD MED SCH,ICRF ONCOL UNIT,LONDON W12 0NN,ENGLAND. ACAD HOSP JETTE,DEPT PATHOL,B-1090 BRUSSELS,BELGIUM. HAMMERSMITH HOSP,ROYAL POSTGRAD MED SCH,DEPT SURG,LONDON W12 0NN,ENGLAND.
Int J Oncol. 1994 Jun;4(6):1219-23. doi: 10.3892/ijo.4.6.1219.
Growth factors may play a role in autocrine or paracrine growth control of tumour cells. We have now examined the expression pattern in vivo by in situ hybridization (ISH) on a series of 13 ductal adenocarcinomas of the pancreas using the non-radioactive digoxigenin system to generate specific antisense orientated riboprobes for FGF-1 and FGF-2, and the four FGFRs (FGFR-1, -2, -3 and -4). We confirmed the expression of both FGF/FGFR by tumour cells, with the potential of a potential autocrine loops in 46% of the cases studied. FGF-2 and FGFR-3 were the most commonly expressed ligand and receptor (46% and 76% respectively). Endothelial cells lining vessels within an around invasive tumours were frequently positive for expression of FGFR-1 and/or FGFR-3. In the normal pancreas remote from the tumour, the acinar cells were found to have a heterogeneous expression pattern for FGFRs while duct cells, islet cells and stromal components including nerves and vascular endothelium were negative. The data suggest a role for FGFs and their high affinity receptors in the control of growth of human pancreatic adenocarcinoma and its supporting stroma.
生长因子可能在肿瘤细胞的自分泌或旁分泌生长控制中发挥作用。我们现在通过原位杂交（ISH），使用非放射性地高辛配体系统，针对13例胰腺导管腺癌系列样本，生成FGF - 1、FGF - 2以及四种FGFR（FGFR - 1、- 2、- 3和- 4）的特异性反义定向核糖探针，来研究体内的表达模式。我们证实肿瘤细胞表达FGF/FGFR，在所研究的病例中，46%存在潜在的自分泌环。FGF - 2和FGFR - 3是最常表达的配体和受体（分别为46%和76%）。侵袭性肿瘤内部及周围血管的内皮细胞FGFR - 1和/或FGFR - 3表达常常呈阳性。在远离肿瘤的正常胰腺中，发现腺泡细胞FGFRs表达模式具有异质性，而导管细胞、胰岛细胞以及包括神经和血管内皮在内的间质成分均为阴性。这些数据表明FGFs及其高亲和力受体在人类胰腺腺癌及其支持性间质的生长控制中发挥作用。
