Department of Surgical Oncology, Zhejiang Province Cancer Hospital, Hangzhou 310022, PR China.
Oncol Rep. 2011 Aug;26(2):349-57. doi: 10.3892/or.2011.1282. Epub 2011 Apr 28.
To elucidate the potential etiological role of RUNX3 in the development of salivary gland adenoid cystic carcinoma (ACC), we analyzed the methylation status of RUNX3 in a series of 114 ACC tissues and 3 ACC cell lines. Results showed that the methylated rate of RUNX3 was 50.9 and 3.5% in the 114 ACC samples and the corresponding normal salivary glands, respectively, achieving a significant difference (P<0.001). There was a significant correlation between RUNX3 methylation and various clinicopathological parameters of ACCs, such as perineural invasion, lymph node involvement, T-stage and distant metastasis (P<0.001). RUNX3 methylation was a significant predictor of the 5-year disease-free survival in ACC patients after surgery. Partial methylation was found in all 3 ACC cell lines, and the reactivation and more potent expression of RUNX3 was induced by 5-triazole-2-deoxycytidine. Our findings indicate that RUNX3 methylation may occur as a common event in the development of ACC and that methylation may be a major mechanism for inactivation of RUNX3 in ACC.
为了阐明 RUNX3 在唾液腺腺样囊性癌（ACC）发生发展中的潜在病因学作用，我们分析了 114 例 ACC 组织和 3 种 ACC 细胞系中 RUNX3 的甲基化状态。结果显示，在 114 例 ACC 样本和相应的正常唾液腺中，RUNX3 的甲基化率分别为 50.9%和 3.5%，差异具有统计学意义（P<0.001）。RUNX3 甲基化与 ACC 的各种临床病理参数，如神经周围侵犯、淋巴结转移、T 分期和远处转移均存在显著相关性（P<0.001）。RUNX3 甲基化是 ACC 患者术后 5 年无病生存率的显著预测因子。在所有 3 种 ACC 细胞系中均发现了部分甲基化，5-三唑-2-脱氧胞苷可诱导 RUNX3 的重新激活和更强烈的表达。我们的研究结果表明，RUNX3 甲基化可能是 ACC 发展中的一个常见事件，而甲基化可能是 ACC 中 RUNX3 失活的主要机制。
