Nasi S, Sirinian M I, Panetta G, Marchetti A, Jucker R
Centro per lo Studio degli Acidi Nucleici CNR, Dipartimento di Genetica e Biologia Molecolare, Universitá La Sapienza, A. Moro, Rome, Italy.
Oncogene. 1990 Jan;5(1):117-22.
We report on the use of human B lymphocytes immortalized by the Epstein-Barr virus (EBV) as targets for transformation by the c-Ha-ras oncogene of bladder carcinoma cells T24. Several stably transformed cell lines were obtained and their in vivo and in vitro growth properties as well as levels of expression of the ras gene were studied. The transformed phenotype in these cells was correlated to ras oncoprotein expression level; only the cell lines which overproduce p21 ras, by at least six-fold, were tumorigenic in nude mice. In this regard, our ras transformed cells behave as lymphoblastoid cells transformed by the c-myc oncogene, suggesting that c-myc and c-Ha-ras might act on the same regulatory level.
我们报道了将经爱泼斯坦-巴尔病毒（EBV）永生化的人B淋巴细胞作为膀胱癌细胞T24的c-Ha-ras癌基因转化的靶细胞。获得了几种稳定转化的细胞系，并研究了它们在体内和体外的生长特性以及ras基因的表达水平。这些细胞中的转化表型与ras癌蛋白表达水平相关；只有那些p21 ras过度表达至少6倍的细胞系在裸鼠中具有致瘤性。在这方面，我们的ras转化细胞表现得如同被c-myc癌基因转化的淋巴母细胞，这表明c-myc和c-Ha-ras可能在相同的调控水平上起作用。
