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一种从基因组文库中快速高效检测 SSR 的生物信息学工具。

cid: a rapid and efficient bioinformatic tool for the detection of SSRs from genomic libraries.

机构信息

Departamento de Genética e Evolução, Universidade Federal de São Carlos, Via Washington Luiz km 235, Caixa Postal 676, CEP 13565-905, São Carlos, SP, Brazil.

出版信息

Mol Ecol Resour. 2008 Jan;8(1):107-8. doi: 10.1111/j.1471-8286.2007.01950.x.

Abstract

cid is a computational tool developed in the Web environment to process cloned DNA fragments with the objective of masking the vector and adaptor regions, detecting the presence of microsatellites and designing the most appropriate primer pairs for the amplification of the identified repetitive sequences. This entire process is executed by the user in a simple and automated manner with the data input as a Zip file of chromatograms or a multiFASTA file. Thus, it is possible to analyse dozens of sequences at the same time, optimizing data processing and the search for the information of interest. cid is freely available on http://www.shrimp.ufscar.br/cid/index.php.

摘要

cid 是一个在网络环境中开发的计算工具,用于处理克隆的 DNA 片段,目的是屏蔽载体和接头区域,检测微卫星的存在,并为鉴定的重复序列设计最合适的引物对。整个过程由用户以一种简单和自动化的方式执行,输入的数据为色谱图的 Zip 文件或多 FASTA 文件。因此,可以同时分析几十个序列,优化数据处理和感兴趣信息的搜索。cid 可在 http://www.shrimp.ufscar.br/cid/index.php 免费获得。

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