红榆（Ulmus rubra Muhl.）微卫星标记的分离与鉴定及其与西伯利亚榆（Ulmus pumila L.）的跨物种扩增。

Isolation and characterization of microsatellite markers for red elm (Ulmus rubra Muhl.) and cross-species amplification with Siberian elm (Ulmus pumila L.).

机构信息

Agricultural Research Service, Vegetable Crops Unit, Department of Horticulture, US Department of Agriculture, University of Wisconsin, Madison, 1575 Linden Drive, WI 53706 USA, Department of Forest Ecology and Management, University of Wisconsin, Madison, 1630 Linden Drive, WI 53706, USA.

出版信息

Mol Ecol Resour. 2008 Jan;8(1):109-12. doi: 10.1111/j.1471-8286.2007.01805.x.

DOI:10.1111/j.1471-8286.2007.01805.x

PMID:21585729

Abstract

Ulmus pumila is an elm species, non-native to the USA that hybridizes with Ulmus rubra. In order to study the genetic structure and hybridization patterns between these two elm species, we developed 15 primer pairs for microsatellite loci in U. rubra and tested their cross-amplification in U. pumila. All 15 primers amplified in both species, 11 of which possessed species-specific alleles. Eight loci were polymorphic in U. pumila and eight in U. rubra, each with two to eight alleles per locus. In addition, five primer pairs previously developed in U. laevis and U. carpinifolia (syn. U. minor) cross-amplified and showed polymorphic loci in U. pumila and/or U. rubra. These markers will facilitate the study of genetic structure and gene flow between U. rubra and exotic, invasive U. pumila.

摘要

榆树是一种非本地原产于美国的榆树种，与美国红榆杂交。为了研究这两个榆树种之间的遗传结构和杂交模式，我们开发了 15 对微卫星标记引物用于 U. rubra，并测试了它们在 U. pumila 中的交叉扩增。这 15 对引物在两个物种中都能扩增，其中 11 对具有种特异性等位基因。8 个位点在 U. pumila 中多态性，8 个在 U. rubra 中多态性，每个位点有 2 到 8 个等位基因。此外，之前在 U. laevis 和 U. carpinifolia（syn. U. minor）中开发的 5 对引物对在 U. pumila 和/或 U. rubra 中也能扩增并显示出多态性位点。这些标记将有助于研究 U. rubra 和外来入侵的 U. pumila 之间的遗传结构和基因流。