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从大西洋雨林特有凤梨科植物白花皮氏凤梨中分离和特征化微卫星位点,以及在其他物种中的交叉扩增。

Isolation and characterization of microsatellite loci in Pitcairnia albiflos (Bromeliaceae), an endemic bromeliad from the Atlantic Rainforest, and cross-amplification in other species.

机构信息

Departamento de Genética, IB/UFRGS, 91501-970 Porto Alegre, RS, Brazil, Departamento de Genética e Evolução, IB/UNICAMP, 13083-970 Campinas, SP, Brazil, Centro de Biologia Molecular e Engenharia Genética (CBMEG), UNICAMP, 13083-970 Campinas, SP, Brazil, Departamento de Botânica, CCS/IB/UFRJ, 21941-590 Rio de Janeiro, RJ, Brazil, Jodrell Laboratory, Royal Botanic Gardens Kew, Richmond, Surrey TW9 3DS, UK.

出版信息

Mol Ecol Resour. 2008 Sep;8(5):980-2. doi: 10.1111/j.1755-0998.2008.02126.x. Epub 2008 Jun 28.

DOI:10.1111/j.1755-0998.2008.02126.x
PMID:21585947
Abstract

Eight microsatellite markers were isolated from Pitcairnia albiflos, an endangered endemic bromeliad species restricted to 'inselberg' rock outcrops in the state of Rio de Janeiro, Brazil. The number of alleles observed for each locus ranged from two to 12. Average observed and expected heterozygosities were 0.408 and 0.663, respectively. The cross-amplification test in 16 taxa suggests that the markers will be useful in numerous related bromeliad species. The loci will be used to study genetic structure and reproductive biology in fragmented inselberg populations and the origin and maintenance of barriers to gene flow between sympatric Pitcairnia species.

摘要

从巴西里约热内卢州仅限于“岩岛”露头的濒危特有凤梨科植物白花皮氏凤梨中分离出 8 个微卫星标记。每个位点观察到的等位基因数从 2 到 12 不等。平均观察到的和预期的杂合度分别为 0.408 和 0.663。在 16 个分类群中的交叉扩增测试表明,这些标记将对许多相关的凤梨科物种有用。这些位点将用于研究碎片化岩岛种群的遗传结构和繁殖生物学,以及同域皮氏凤梨属物种之间基因流动障碍的起源和维持。

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