开发用于鲤鱼种群研究的 10 个三核苷酸和四核苷酸微卫星位点。

Development of 10 tri- and tetranucleotide microsatellite loci for population studies in the common carp (Cyprinus carpio L.).

机构信息

Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China, College of Aqua-life Science and Technology, Dalian Fisheries University, Dalian 116023, China, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China.

出版信息

Mol Ecol Resour. 2008 Nov;8(6):1357-9. doi: 10.1111/j.1755-0998.2008.02305.x. Epub 2008 Aug 25.

DOI:10.1111/j.1755-0998.2008.02305.x

PMID:21586045

Abstract

Ten tri- and tetranucleotide microsatellite DNA markers were isolated and characterized from common carp (Cyprinus carpio L.) to estimate genetic potential. These markers were tested in the samples from two closely related carp populations (Cyprinus carpio var. xingguonensis and Cyprinus carpio var. wananensis). The number of the alleles ranged from three to nine, and observed and expected hererozygosities varied from 0.207 to 1.000 and from 0.499 to 0.900 in each population, respectively. No evidence for linkage disequilibrium was found, indicating that these markers will be useful for population studies.

摘要

从鲤鱼（Cyprinus carpio L.）中分离并鉴定了 10 个三核苷酸和四核苷酸微卫星 DNA 标记，以评估遗传潜力。在两个亲缘关系密切的鲤鱼种群（兴国红鲤和万安红鲤）的样本中对这些标记进行了测试。等位基因数从 3 到 9 不等，每个种群的观察和预期杂合度分别在 0.207 到 1.000 和 0.499 到 0.900 之间。未发现连锁不平衡的证据，表明这些标记将对种群研究有用。