新一代测序技术及其在真菌基因组学中的潜在应用。

Next-generation sequencing and potential applications in fungal genomics.

作者信息

Sanmiguel Phillip

机构信息

Purdue Genomics Core Facility, Purdue University, West Lafayette, IN, USA.

出版信息

Methods Mol Biol. 2011;722:51-60. doi: 10.1007/978-1-61779-040-9_4.

DOI:10.1007/978-1-61779-040-9_4

PMID:21590412

Abstract

Since the first fungal genome was sequenced in 1996, sequencing technologies have advanced dramatically. In recent years, it has become possible to cost-effectively generate vast amounts of DNA sequence data using a number of cell- and electrophoresis-free sequencing technologies, commonly known as "next" or "second" generation. In this chapter, we present a brief overview of next-generation sequencers that are commercially available now. Their potential applications in fungal genomics studies are discussed.

摘要

自1996年首个真菌基因组测序完成以来，测序技术取得了巨大进展。近年来，使用多种无细胞和无电泳的测序技术（通常称为“下一代”或“第二代”测序技术），以具有成本效益的方式生成大量DNA序列数据已成为可能。在本章中，我们简要概述了目前市面上可买到的下一代测序仪，并讨论了它们在真菌基因组学研究中的潜在应用。