用于研究栗疫病菌-低毒病毒实验系统的分子方法。

Molecular methods for studying the Cryphonectria parasitica - hypovirus experimental system.

作者信息

Dawe Angus L, Mu Rong, Rivera Gloricelys, Salamon Joanna A

机构信息

Department of Biology and Molecular Biology Program, New Mexico State University, Las Cruces, NM, USA.

出版信息

Methods Mol Biol. 2011;722:225-36. doi: 10.1007/978-1-61779-040-9_17.

DOI:10.1007/978-1-61779-040-9_17

PMID:21590425

Abstract

The interaction of the filamentous fungal plant pathogen Cryphonectria parasitica with its virulence-attenuating viruses provides a unique platform to explore the molecular biology and genetics of virus-host interactions. Following the development of transformation procedures for this fungus, subsequent advances include infectious cDNA clones of several members of the Hypoviridae and an imminently complete fungal genome project. Presented here are basic protocols for growth of the organism and the extraction of DNA, RNA, and protein. Additionally, two further protocols are provided for investigations of host protein phosphorylation and for viral genome secondary structure.

摘要

丝状真菌植物病原体寄生隐孢壳菌与其减毒病毒的相互作用为探索病毒-宿主相互作用的分子生物学和遗传学提供了一个独特的平台。随着该真菌转化程序的发展，后续进展包括几个病毒科成员的感染性cDNA克隆以及即将完成的真菌基因组计划。本文介绍了该生物体生长以及DNA、RNA和蛋白质提取的基本方案。此外，还提供了另外两个方案，用于研究宿主蛋白磷酸化和病毒基因组二级结构。