Wiederhold K H, Palacios J M
Sandoz Ltd, Basle, Switzerland.
J Microsc. 1990 Mar;157(Pt 3):335-47. doi: 10.1111/j.1365-2818.1990.tb02969.x.
The use of the receptor autoradiography and 2-deoxyglucose (2-DG) techniques in neuroscience are reviewed. Receptors and other binding sites can be visualized autoradiographically in microtome tissue sections after labelling with radioligand in vivo or in vitro. Autoradiograms are generated by apposition of the labelled tissue to photographic emulsions. Combined with computerized image analysis, this technique can be used to analyse and quantify the microscopic distribution of receptors and receptor alterations associated with lesions or disease in human and animal tissues. The 2-DG technique permits microscopic analysis of modifications in brain glucose utilization induced by physiological and pharmacological manipulations. Limitations of these techniques and attempts to optimize their resolution are also discussed.
本文综述了受体放射自显影术和2-脱氧葡萄糖（2-DG）技术在神经科学中的应用。在用放射性配体进行体内或体外标记后，可在切片组织中通过放射自显影术观察到受体和其他结合位点。放射自显影片是通过将标记组织与感光乳剂贴合而产生的。结合计算机图像分析，该技术可用于分析和量化人类和动物组织中受体的微观分布以及与损伤或疾病相关的受体改变。2-DG技术可对生理和药理操作引起的脑葡萄糖利用变化进行微观分析。文中还讨论了这些技术的局限性以及优化其分辨率的尝试。
