N-酰基化 l-高丝氨酸内酯衍生亲和基质的合成及其在群体感应信号受体分离中的应用。
Synthesis and application of an N-acylated l-homoserine lactone derivatized affinity matrix for the isolation of quorum sensing signal receptors.
机构信息
Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, Madison, WI 53706-1322, USA.
出版信息
Bioorg Med Chem Lett. 2011 Sep 1;21(17):5054-7. doi: 10.1016/j.bmcl.2011.04.062. Epub 2011 Apr 22.
Abstract
The design and synthesis of an agarose resin functionalized with a Gram-negative quorum sensing (QS) signaling molecule analogue is described. The modified resin was utilized in affinity pull-down assays to successfully isolate QscR, a LuxR-type QS receptor from Pseudomonas aeruginosa. This resin may facilitate the identification of novel QS signal receptors using affinity chromatography techniques.
摘要
本文描述了一种琼脂糖树脂的设计和合成，该树脂功能化了革兰氏阴性群体感应 (QS) 信号分子类似物。改性树脂用于亲和下拉测定中，成功地从铜绿假单胞菌中分离出了 QscR，一种 LuxR 型 QS 受体。这种树脂可能会使用亲和层析技术促进新型 QS 信号受体的鉴定。
相似文献
1
Synthesis and application of an N-acylated l-homoserine lactone derivatized affinity matrix for the isolation of quorum sensing signal receptors.N-酰基化 l-高丝氨酸内酯衍生亲和基质的合成及其在群体感应信号受体分离中的应用。
Bioorg Med Chem Lett. 2011 Sep 1;21(17):5054-7. doi: 10.1016/j.bmcl.2011.04.062. Epub 2011 Apr 22.
2
A comparative study of non-native N-acyl l-homoserine lactone analogs in two Pseudomonas aeruginosa quorum sensing receptors that share a common native ligand yet inversely regulate virulence.两种铜绿假单胞菌群体感应受体中，非天然 N-酰基高丝氨酸内酯类似物的比较研究，这两种受体共享一个共同的天然配体，但却相反地调节毒力。
Bioorg Med Chem. 2018 Oct 15;26(19):5336-5342. doi: 10.1016/j.bmc.2018.05.018. Epub 2018 May 14.
3
The Orphan Quorum Sensing Signal Receptor QscR Regulates Global Quorum Sensing Gene Expression by Activating a Single Linked Operon.孤儿群体感应信号受体 QscR 通过激活单个连锁操纵子来调节全局群体感应基因表达。
mBio. 2018 Aug 28;9(4):e01274-18. doi: 10.1128/mBio.01274-18.
4
Potent and Selective Modulation of the RhlR Quorum Sensing Receptor by Using Non-native Ligands: An Emerging Target for Virulence Control in Pseudomonas aeruginosa.利用非天然配体对RhlR群体感应受体进行强效和选择性调控：铜绿假单胞菌毒力控制的新靶点
Chembiochem. 2015 Nov 2;16(16):2348-56. doi: 10.1002/cbic.201500357. Epub 2015 Oct 13.
5
Synthesis and Biological Evaluation of Novel L-Homoserine Lactone Analogs as Quorum Sensing Inhibitors of Pseudomonas aeruginosa.新型L-高丝氨酸内酯类似物作为铜绿假单胞菌群体感应抑制剂的合成及生物学评价
Chem Pharm Bull (Tokyo). 2019;67(10):1088-1098. doi: 10.1248/cpb.c19-00359.
6
Synthesis and molecular modeling provide insight into a Pseudomonas aeruginosa quorum sensing conundrum.合成与分子建模深入了解铜绿假单胞菌群体感应难题。
J Am Chem Soc. 2011 Mar 23;133(11):3840-2. doi: 10.1021/ja111138y. Epub 2011 Feb 24.
7
An evolving perspective on the Pseudomonas aeruginosa orphan quorum sensing regulator QscR.对铜绿假单胞菌孤儿群体感应调节因子QscR的一种不断演变的观点。
Front Cell Infect Microbiol. 2014 Oct 28;4:152. doi: 10.3389/fcimb.2014.00152. eCollection 2014.
8
Microwave synthesis and evaluation of phenacylhomoserine lactones as anticancer compounds that minimally activate quorum sensing pathways in Pseudomonas aeruginosa.苯甲酰高丝氨酸内酯作为抗癌化合物的微波合成及评估，该化合物对铜绿假单胞菌群体感应途径的激活作用最小。
J Med Chem. 2009 Mar 26;52(6):1569-75. doi: 10.1021/jm8015377.
9
Structural determinants driving homoserine lactone ligand selection in the LasR quorum-sensing receptor.结构决定因素驱动 LasR 群体感应受体中高丝氨酸内酯配体的选择。
Proc Natl Acad Sci U S A. 2019 Jan 2;116(1):245-254. doi: 10.1073/pnas.1817239116. Epub 2018 Dec 17.
10
Efficient synthesis and evaluation of quorum-sensing modulators using small molecule macroarrays.利用小分子宏阵列高效合成及评估群体感应调节剂
Org Lett. 2009 Oct 15;11(20):4600-3. doi: 10.1021/ol901871y.
引用本文的文献
1
Quorum sensing communication between bacteria and human cells: signals, targets, and functions.细菌与人类细胞之间的群体感应通讯：信号、靶标和功能。
Front Plant Sci. 2014 Jun 26;5:309. doi: 10.3389/fpls.2014.00309. eCollection 2014.
2
Design and synthesis of a biotinylated chemical probe for detecting the molecular targets of an inhibitor of the production of the Pseudomonas aeruginosa virulence factor pyocyanin.设计并合成一种生物素化的化学探针，用于检测铜绿假单胞菌毒力因子绿脓菌素产生抑制剂的分子靶标。
Molecules. 2013 Sep 25;18(10):11783-96. doi: 10.3390/molecules181011783.
3
Species selective diazirine positioning in tag-free photoactive quorum sensing probes.无标签光活性群体感应探针中物种选择性重氮化合物定位。
Chem Commun (Camb). 2013 Jul 4;49(52):5826-8. doi: 10.1039/c3cc43092h. Epub 2013 May 24.
4
The Pseudomonas aeruginosa N-acylhomoserine lactone quorum sensing molecules target IQGAP1 and modulate epithelial cell migration.铜绿假单胞菌 N-酰基高丝氨酸内酯群体感应分子靶向 IQGAP1 并调节上皮细胞迁移。
PLoS Pathog. 2012;8(10):e1002953. doi: 10.1371/journal.ppat.1002953. Epub 2012 Oct 11.
5
Chemical methods to interrogate bacterial quorum sensing pathways.化学方法探究细菌群体感应途径。
Org Biomol Chem. 2012 Oct 3;10(41):8189-99. doi: 10.1039/c2ob26353j.
本文引用的文献
1
Quorum sensing in Gram-negative bacteria: small-molecule modulation of AHL and AI-2 quorum sensing pathways.革兰氏阴性菌中的群体感应：AHL和AI-2群体感应途径的小分子调控
Chem Rev. 2011 Jan 12;111(1):28-67. doi: 10.1021/cr100109t. Epub 2010 Dec 23.
2
Structural basis of acyl-homoserine lactone-dependent signaling.酰基高丝氨酸内酯依赖性信号传导的结构基础。
Chem Rev. 2011 Jan 12;111(1):68-85. doi: 10.1021/cr1000817. Epub 2010 Dec 2.
3
Acyl-homoserine lactone binding to and stability of the orphan Pseudomonas aeruginosa quorum-sensing signal receptor QscR.酰基高丝氨酸内酯与孤儿假单胞菌群体感应信号受体 QscR 的结合及其稳定性。
J Bacteriol. 2011 Jan;193(2):421-8. doi: 10.1128/JB.01041-10. Epub 2010 Nov 19.
4
Macromolecular inhibition of quorum sensing: enzymes, antibodies, and beyond.群体感应的大分子抑制作用：酶、抗体及其他。
Chem Rev. 2011 Jan 12;111(1):195-208. doi: 10.1021/cr100101c. Epub 2010 Nov 18.
5
Discovery of a quorum sensing modulator pharmacophore by 3D small-molecule microarray screening.通过 3D 小分子微阵列筛选发现群体感应调节剂药效团。
Org Biomol Chem. 2010 Dec 7;8(23):5313-23. doi: 10.1039/c0ob00300j. Epub 2010 Sep 30.
6
Small molecules that modulate quorum sensing and control virulence in Pseudomonas aeruginosa.调节铜绿假单胞菌群体感应并控制其毒力的小分子。
J Org Chem. 2010 Oct 15;75(20):6737-46. doi: 10.1021/jo101237e.
7
Medicinal chemistry as a conduit for the modulation of quorum sensing.作为群体感应调节途径的药物化学
J Med Chem. 2010 Nov 11;53(21):7467-89. doi: 10.1021/jm901742e.
8
What's in a name? The semantics of quorum sensing.名字有何意义？群体感应的语义学。
Trends Microbiol. 2010 Sep;18(9):383-7. doi: 10.1016/j.tim.2010.05.003. Epub 2010 Jun 21.
9
Perception and degradation of N-acyl homoserine lactone quorum sensing signals by mammalian and plant cells.哺乳动物细胞和植物细胞对N-酰基高丝氨酸内酯群体感应信号的感知与降解
Chem Rev. 2011 Jan 12;111(1):100-16. doi: 10.1021/cr100045m. Epub 2010 Jun 10.
10
In vitro screens for quorum sensing inhibitors and in vivo confirmation of their effect.体外筛选群体感应抑制剂及体内确认其效果。
Nat Protoc. 2010 Feb;5(2):282-93. doi: 10.1038/nprot.2009.205. Epub 2010 Jan 28.
Zotero 插件