N-酰基化 l-高丝氨酸内酯衍生亲和基质的合成及其在群体感应信号受体分离中的应用。
Synthesis and application of an N-acylated l-homoserine lactone derivatized affinity matrix for the isolation of quorum sensing signal receptors.
机构信息
Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, Madison, WI 53706-1322, USA.
出版信息
Bioorg Med Chem Lett. 2011 Sep 1;21(17):5054-7. doi: 10.1016/j.bmcl.2011.04.062. Epub 2011 Apr 22.
Abstract
The design and synthesis of an agarose resin functionalized with a Gram-negative quorum sensing (QS) signaling molecule analogue is described. The modified resin was utilized in affinity pull-down assays to successfully isolate QscR, a LuxR-type QS receptor from Pseudomonas aeruginosa. This resin may facilitate the identification of novel QS signal receptors using affinity chromatography techniques.
摘要
本文描述了一种琼脂糖树脂的设计和合成，该树脂功能化了革兰氏阴性群体感应 (QS) 信号分子类似物。改性树脂用于亲和下拉测定中，成功地从铜绿假单胞菌中分离出了 QscR，一种 LuxR 型 QS 受体。这种树脂可能会使用亲和层析技术促进新型 QS 信号受体的鉴定。
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