Respiratory Medicine and Allergology, Department of Clinical Sciences in Lund, Lund University, Lund, Sweden.
Prostaglandins Leukot Essent Fatty Acids. 2011 Aug;85(2):67-73. doi: 10.1016/j.plefa.2011.04.025. Epub 2011 May 18.
Leukotrienes are involved in airway inflammation, and are believed to stimulate airway remodeling in asthma. The aim of the project was to investigate the expression of leukotriene receptors in peripheral and central airway fibroblasts. Peripheral and central airway fibroblasts, from asthmatics and healthy controls, were investigated for the amount of cysteinyl-leukotriene receptors (CysLT(1) and CysLT(2)), leukotriene B(4) receptors (BLT(1) and BLT(2)), IL-13 receptor-α(1) (IL-13Rα(1)) and the IL-4 receptor (IL-4R). The mRNA expression of CysLT(1) in fibroblasts from peripheral airways was higher compared to central airways. There was no difference in CysLT(2) between peripheral and central airways. On the contrary, BLT(1) and BLT(2) were lower in fibroblasts from peripheral airways compared to central. The expression of CysLT(1) was higher than CysLT(2) in fibroblasts from peripheral airways, and the expression of BLT(1) was higher than BLT(2) in both peripheral and central airways. Both BLT(1) and BLT(2) were higher in asthmatics compared to healthy controls, while CysLT(1) and CysLT(2) did not differ. The expression of IL-13Rα(1) was higher in asthmatics compared to controls, and correlated to the BLTs. All fibroblasts stained for the different receptor proteins. Leukotriene receptors are differently expressed in fibroblasts from peripheral compared to central airways, which may explain a suggested cysteinyl-leukotriene driven remodeling mainly in the peripheral airways.
白三烯参与气道炎症，并被认为刺激哮喘中的气道重塑。该项目的目的是研究外周和中央气道成纤维细胞中白三烯受体的表达。从哮喘患者和健康对照者的外周和中央气道成纤维细胞中，研究了半胱氨酰白三烯受体（CysLT（1）和 CysLT（2））、白三烯 B（4）受体（BLT（1）和 BLT（2））、IL-13 受体-α（1）（IL-13Rα（1））和 IL-4 受体（IL-4R）的数量。与中央气道相比，外周气道成纤维细胞中 CysLT（1）的 mRNA 表达更高。外周和中央气道之间 CysLT（2）没有差异。相反，与中央气道相比，外周气道成纤维细胞中的 BLT（1）和 BLT（2）较低。外周气道成纤维细胞中 CysLT（1）的表达高于 CysLT（2），而 BLT（1）和 BLT（2）在外周和中央气道中的表达均高于 BLT（2）。与健康对照组相比，哮喘患者的 BLT（1）和 BLT（2）表达均升高，而 CysLT（1）和 CysLT（2）则无差异。与对照组相比，哮喘患者的 IL-13Rα（1）表达更高，并且与 BLTs 相关。所有成纤维细胞均对不同的受体蛋白进行染色。与中央气道相比，外周气道的成纤维细胞中白三烯受体的表达不同，这可能解释了一种建议的半胱氨酰白三烯驱动的重塑主要发生在外周气道。
