通过增殖细胞核抗原（PCNA）和核仁组成区嗜银蛋白（AgNORs）评估口腔白斑和鳞状细胞癌的增殖活性。

The estimation of proliferative activity by pcna and AgNORs in leukoplakia and squamous-cell carcinoma of the oral cavity.

作者信息

Kanekawa A, Tsuji T, Mimura Y, Murakami T, Li X, Wen S, Shinozaki F

机构信息

YAMAGUCHI UNIV,SCH MED,DEPT ORAL & MAXILLOFACIAL SURG,UBE,YAMAGUCHI 755,JAPAN. YAMAGUCHI PREFECTURAL CENT HOSP,DIV ORAL & MAXILLOFACIAL SURG,HOFU,YAMAGUCHI 747,JAPAN. YAMAGUCHI UNIV,SCH MED,DEPT PATHOL 2,UBE,YAMAGUCHI 755,JAPAN. CHINA MED UNIV,SCH MED,DEPT ORAL PATHOL,SHENYANG 110002,PEOPLES R CHINA. CHINA MED UNIV,SCH MED,DEPT ORAL & MAXILLOFACIAL SURG,SHENYANG 110002,PEOPLES R CHINA.

出版信息

Oncol Rep. 1995 Sep;2(5):711-5. doi: 10.3892/or.2.5.711.

DOI:10.3892/or.2.5.711

PMID:21597802

Abstract

The proliferating activity of leukoplakia and squamous cell carcinoma (SCC) was estimated using proliferating cell nuclear antigen (PCNA) immunostaining and silver-binding argyrophilic nucleolar organizer region (AgNOR) staining. Twenty-eight leukoplakias and 15 SCCs of the oral cavity were used in this study. The mean+/-S.D. of PCNA LI and AgNOR counts were 7.7+/-6.0% and 2.43+/-0.68/nucleus in leukoplakias, and 22.3+/-11.6% and 4.77+/-1.49/nucleus in SCCs. Both of PCNA LI and AgNOR counts were significantly higher in SCCs than in leukoplakias. There was a significant linear correlation between PCNA LI and AgNOR counts (p=0.0022) in leukoplakias, but not in SCCs. In the series of leukoplakias, PCNA LI apparently increased in leukoplakias with dysplasia and malignant transformed cases. Our data suggest that PCNA LI and AgNOR counts are useful markers of proliferating activity, and PCNA LI might be a prognostic factor of leukoplakias.

摘要

采用增殖细胞核抗原（PCNA）免疫染色和银结合嗜银核仁组织区（AgNOR）染色法评估口腔白斑和鳞状细胞癌（SCC）的增殖活性。本研究使用了28例口腔白斑和15例口腔SCC。白斑中PCNA标记指数（LI）和AgNOR计数的平均值±标准差分别为7.7±6.0%和2.43±0.68/核，SCC中分别为22.3±11.6%和4.77±1.49/核。SCC中的PCNA LI和AgNOR计数均显著高于白斑。白斑中PCNA LI与AgNOR计数之间存在显著的线性相关性（p = 0.0022），而SCC中则无。在白斑系列中，PCNA LI在伴有发育异常和恶变的白斑中明显增加。我们的数据表明，PCNA LI和AgNOR计数是增殖活性的有用标志物，PCNA LI可能是白斑的一个预后因素。