Sub-department of Environmental Technology, Wageningen University, Bomenweg 2, Wageningen, The Netherlands.
Bioresour Technol. 2011 Aug;102(15):7257-64. doi: 10.1016/j.biortech.2011.04.095. Epub 2011 May 1.
The present research demonstrates the biological treatment of refinery sulfidic spent caustics in a continuously fed system under halo-alkaline conditions (i.e. pH 9.5; Na(+)= 0.8M). Experiments were performed in identical gas-lift bioreactors operated under aerobic conditions (80-90% saturation) at 35°C. Sulfide loading rates up to 27 mmol L(-1)day(-1) were successfully applied at a HRT of 3.5 days. Sulfide was completely converted into sulfate by the haloalkaliphilic sulfide-oxidizing bacteria belonging to the genus Thioalkalivibrio. Influent benzene concentrations ranged from 100 to 600 μM. At steady state, benzene was removed by 93% due to high stripping efficiencies and biodegradation. Microbial community analysis revealed the presence of haloalkaliphilic heterotrophic bacteria belonging to the genera Marinobacter, Halomonas and Idiomarina which might have been involved in the observed benzene removal. The work shows the potential of halo-alkaliphilic bacteria in mitigating environmental problems caused by alkaline waste.
本研究在亚碱性条件(即 pH 值 9.5;Na(+) = 0.8M)下,在连续进料系统中展示了炼油厂含硫废碱的生物处理。实验在相同的好气(80-90%饱和度)气体升力生物反应器中在 35°C 下进行。在 HRT 为 3.5 天时,成功应用了高达 27 mmol L(-1)day(-1)的硫化物负荷率。亚碱性硫氧化细菌属的硫氧化细菌将硫化物完全转化为硫酸盐。苯的入口浓度范围为 100 至 600 μM。在稳定状态下,由于高汽提效率和生物降解,苯去除率达到 93%。微生物群落分析表明,存在属于 Marinobacter、Halomonas 和 Idiomarina 的耐碱异养细菌,它们可能参与了观察到的苯去除。这项工作表明了耐碱嗜盐细菌在减轻碱性废物造成的环境问题方面的潜力。