[编码HIV-1 B亚型env基因的质粒DNA和腺病毒载体的免疫原性]
[Immunogenicity of plasmid DNA and adenoviral vectors encoding HIV-1 subtype B env gene].
作者信息
Yang Hai-Ru, Zhang Ling-Fei, Feng Xia, Yu Shuang-Qing, Zhuang Zhu-Lun, Li Hong-Xia, Zeng Yi
机构信息
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, China.
出版信息
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2010 Dec;24(6):415-7.
OBJECTIVE
To construct DNA and recombinant adenovirus vector vaccines containing an env gene from the prevalent subtype B strain in China and try to use them for therapeutic and prophylactic vaccines.
METHODS
The candidate plasmid DNA vaccine pVR-gp160 and recombinant adenovirus vaccine rAdV-gp160 were constructed separately. BALB/c mice were immunized with these two vaccines in different administration schemes. HIV-1 Gp120-specific cellular responses and antibody levels were detected by ELISPOT and ELISA respectively.
RESULTS
DNA vaccine alone and combined vaccines in a DNA prime/rAdV-gp160 boost vaccination regimen induced high level of Gp120-specific cellular responses. While low level of Gp120-specific antibodies were elicited in all groups.
CONCLUSION
DNA and rAdV vaccines could efficiently express Gp160 protein and activate specific cellular responses.
目的
构建含有中国流行B亚型毒株env基因的DNA及重组腺病毒载体疫苗，并尝试将其用作治疗性和预防性疫苗。
方法
分别构建候选质粒DNA疫苗pVR-gp160和重组腺病毒疫苗rAdV-gp160。用这两种疫苗以不同给药方案免疫BALB/c小鼠。分别通过ELISPOT和ELISA检测HIV-1 Gp120特异性细胞反应和抗体水平。
结果
单独的DNA疫苗以及DNA初免/rAdV-gp160加强免疫方案的联合疫苗诱导了高水平的Gp120特异性细胞反应。而所有组中诱导产生的Gp120特异性抗体水平较低。
结论
DNA和rAdV疫苗能够有效表达Gp160蛋白并激活特异性细胞反应。
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