Department of Otorhinolaryngology-Head and Neck Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong SAR, China.
Head Neck. 2012 Apr;34(4):520-33. doi: 10.1002/hed.21772. Epub 2011 May 23.
The purpose of this study of human papillomavirus (HPV), Epstein-Barr virus (EBV), p21, and p53 in sinonasal inverted papilloma (IP) was to help elucidate its pathogenesis.
Seventy-three IPs, 48 nasal polyps, and 85 hypertrophied turbinates were subjected to HPV polymerase chain reaction (PCR) study. Seventy-three IPs, 30 nasal polyps, and 32 hypertrophied turbinates were subjected to EBV in situ hybridization (ISH), p21, and p53 immunohistochemical (IHC) studies.
HPV was positive in 3 of 73 IPs (4.1%). All specimens were EBV negative. In all, 99% of IPs showed strong and diffuse p21 nuclear reactivity. Most nasal polyps and hypertrophied turbinates showed weak to moderate immunoreactivity of the basal and parabasal cells. Only focal p53 immunoreactivity of the basal and parabasal cells was found in 19% of IPs and 40% of nasal polyps.
HPV prevalence of our IP is low. EBV is not present in IP. High p21 and low p53 expression in IP suggests a non-p53-dependent regulation pathway.
本研究旨在探讨人类乳头瘤病毒（HPV）、爱泼斯坦-巴尔病毒（EBV）、p21 和 p53 在鼻窦内翻性乳头状瘤（IP）中的作用，以帮助阐明其发病机制。
对 73 例 IP、48 例鼻息肉和 85 例鼻甲肥大组织进行 HPV 聚合酶链反应（PCR）研究。对 73 例 IP、30 例鼻息肉和 32 例鼻甲肥大组织进行 EBV 原位杂交（ISH）、p21 和 p53 免疫组织化学（IHC）研究。
HPV 在 3 例 IP（4.1%）中呈阳性。所有标本 EBV 均为阴性。99%的 IP 显示出强烈而弥漫的 p21 核反应性。大多数鼻息肉和鼻甲肥大组织的基底细胞和副基底细胞呈弱至中度免疫反应性。仅在 19%的 IP 和 40%的鼻息肉中发现基底细胞和副基底细胞的局灶性 p53 免疫反应性。
我们的 IP 中 HPV 的流行率较低。EBV 不存在于 IP 中。IP 中高表达的 p21 和低表达的 p53 提示存在非 p53 依赖的调节途径。
