高效液相色谱法测定尿中黄曲霉毒素M1的新方法及其在肝癌病因学研究中的应用

A new method for the quantitation of aflatoxin M1 in urine by high performance liquid chromatography and its application to the etiologic study of hepatoma.

作者信息

Liu Z H, Tu W S, Li D R, Li Y D, Xie C H, Yang Y Z, Qin B B

机构信息

Guangxi Cancer Institute, Nanning, China.

出版信息

Biomed Chromatogr. 1990 Mar;4(2):83-6. doi: 10.1002/bmc.1130040210.

DOI:10.1002/bmc.1130040210

PMID:2161690

Abstract

A high performance liquid chromatographic (HPLC) method for the analysis of aflatoxin M1 (AFM1) in urine is described. Urine samples were treated with saturated lead acetate and AFM1 was extracted with chloroform. After washing with water to remove impurities the compound was derivatized with trifluoroacetic acid and the AFM1 derivative was analyzed quantitatively by HPLC. The sample pretreatment is simple and more selective. A good line correlation between AFM1 peak height and its concentration was obtained when AFM1 content was in the range of 50-400 pg. The ratio of recovery was 87.42%. Sensitivity is 0.01 ppb. The method is applicable to trace analysis. Results in urine of residents who live in the high/low liver cancer incidence area in Fushui county were the same as that of previous epidemiological investigation.

摘要

描述了一种用于分析尿液中黄曲霉毒素M1（AFM1）的高效液相色谱（HPLC）方法。尿液样品用饱和醋酸铅处理，AFM1用氯仿萃取。用水洗涤以除去杂质后，该化合物用三氟乙酸衍生化，AFM1衍生物通过HPLC进行定量分析。样品预处理简单且选择性更高。当AFM1含量在50 - 400 pg范围内时，AFM1峰高与其浓度之间具有良好的线性相关性。回收率为87.42%。灵敏度为0.01 ppb。该方法适用于痕量分析。在富水县肝癌高/低发区居民尿液中的检测结果与先前的流行病学调查结果一致。