快速检测血琼脂中硝酸还原酶法检测耐多药结核分枝杆菌。
A rapid detection of multidrug-resistant Mycobacterium tuberculosis by a nitrate reductase assay on blood agar.
机构信息
Department of Medical Microbiology, Medical School, Ondokuz Mayis University, Samsun, Turkey.
出版信息
Mem Inst Oswaldo Cruz. 2011 May;106(3):378-80. doi: 10.1590/s0074-02762011000300022.
The susceptibility of 49 Mycobacterium tuberculosis clinical isolates to isoniazid (INH) and rifampisin (RIF) (28 multi-drug resistant-tuberculosis samples) was determined by a nitrate reductase assay (NRA) on blood agar. Agreement between the NRA and other testing methods was found to be 93.8% for both INH and RIF. The sensitivity, specificity, positive predictive value and negative predictive value for INH were 92.8%, 94.2%, 86.6% and 97%, respectively. The sensitivity, specificity, positive predictive value and negative predictive value for RIF were 90.4%, 96.4%, 95% and 93.1%. In conclusion, we show here that blood agar can be used effectively for the NRA test.
我们采用硝酸还原酶检测法（NRA）在血琼脂平板上对 49 株结核分枝杆菌临床分离株（包括 28 株耐多药结核分枝杆菌样本）的异烟肼（INH）和利福平（RIF）药敏性进行了检测。结果显示，NRA 与其他检测方法对 INH 和 RIF 的符合率分别为 93.8%。INH 的敏感性、特异性、阳性预测值和阴性预测值分别为 92.8%、94.2%、86.6%和 97%。RIF 的敏感性、特异性、阳性预测值和阴性预测值分别为 90.4%、96.4%、95%和 93.1%。总之，本研究表明血琼脂平板可有效地用于 NRA 检测。
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