Haynes Christopher A
Newborn Screening Quality Assurance Program, NSQAP, USA.
Biochim Biophys Acta. 2011 Nov;1811(11):663-8. doi: 10.1016/j.bbalip.2011.05.010. Epub 2011 Jun 6.
Acyl-CoAs are intermediates of numerous metabolic processes in eukaryotic cells, including beta-oxidation within mitochondria and peroxisomes, and the biosynthesis/remodeling of lipids (e.g. mono-, di-, and triglycerides, phospholipids and sphingolipids). Investigations of lipid metabolism have been advanced by the ability to quantitate acyl-CoA intermediates via liquid chromatography coupled to electrospray ionization-tandem mass spectrometric detection (LC-ESI-MS/MS), which is presently one of the most sensitive and specific analytical methods for both lipids and acyl-CoAs. This review of acyl-CoA analysis by mass spectrometry focuses on mammalian samples and long-chain analytes (i.e. palmitoyl-CoA), particularly reports of streamlined methodology, improved recovery, or expansion of the number of acyl chain-lengths amenable to quantitation.
酰基辅酶A是真核细胞中众多代谢过程的中间体，包括线粒体内和过氧化物酶体中的β-氧化，以及脂质（如单酰甘油、二酰甘油和三酰甘油、磷脂和鞘脂）的生物合成/重塑。通过液相色谱与电喷雾电离串联质谱检测（LC-ESI-MS/MS）定量酰基辅酶A中间体的能力推动了脂质代谢的研究，目前这是脂质和酰基辅酶A最灵敏、最特异的分析方法之一。这篇关于质谱分析酰基辅酶A的综述聚焦于哺乳动物样本和长链分析物（即棕榈酰辅酶A），特别报道了简化的方法、提高的回收率或可定量的酰基链长度数量的增加。
