从动物组织中快速分离基因组DNA。
Rapid isolation of genomic DNA from animal tissues.
作者信息
Konat G, Gantt G, Laszkiewicz I, Hogan E L
机构信息
Department of Neurology, Medical University of South Carolina, Charleston.
出版信息
Exp Cell Res. 1990 Oct;190(2):294-6. doi: 10.1016/0014-4827(90)90200-t.
A simple technique for the isolation of very high molecular weight genomic DNA from animal tissues and cells is described. The method involves rapid isolation of nuclei and their embedding in agarose beads followed by extraction of lipids and proteins with SDS. The protocol does not require proteolytic digestion and the whole procedure can be completed in 1 day. The isolated DNA is digestible by restriction enzymes and free of ligase inhibitors.
本文描述了一种从动物组织和细胞中分离非常高分子量基因组DNA的简单技术。该方法包括快速分离细胞核并将其包埋在琼脂糖珠中，然后用SDS提取脂质和蛋白质。该方案不需要蛋白水解消化，整个过程可在1天内完成。分离出的DNA可被限制性内切酶消化且不含连接酶抑制剂。
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