Ramcharan G, Clement Y N, Maxwell A R
Pharmacology Unit, Faculty of Medical Sciences, The University of the West Indies, St Augustine, Trinidad and Tobago, West Indies.
West Indian Med J. 2010 Dec;59(6):597-601.
To assess the cytotoxic activities of crude extracts and solvent fractions of Spermacoce verticillata, Ficus pumila and Flemingia strobilifera against a MT-4 human leukaemia cancer cell line.
Crude extracts of dried leaves of S verticillata, F pumila and F strobilifera were made by exhaustive methanol extraction, fractions were obtained from sequential extraction of the crude extract using solvents of increasing polarity. Dose responses corresponding to cell survival following 72-hour exposure to the extracts were determined using a leukaemia cancer cell line (MT-4). Cell viability was assessed using the MTT[3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide] assay reading absorbances at 570 nm. Comparisons were made with controls and cell survival, in each sample well, was determined based on the ratio of the absorbance of the sample to the control.
Crude extracts of S verticillata, F pumila and F strobilifera displayed cytotoxicity and the IC50 values were 89 microg/ml, 131 microg/ml and 81 microg/ml, respectively. The petroleum ether and chloroform fractions of the crude extracts of S verticillata and F strobilifera showed potent cytotoxic activity but the highest cytotoxic activity was found in the chloroform and butanol fractions of F pumila with IC50 values of 23 microg/ml and 26 microg/ml, respectively.
The crude extracts of S verticillata, F pumila and F strobilifera were shown to be cytotoxic to the leukaemia cell line, MT-4 and IC50 values were determined. Fractionation of the crude extracts by solvent-solvent extraction enabled determination of the active fractions and their IC50 values. We propose that cytotoxic activity may be due to antioxidant compounds previously isolated from these plants.
评估节节花、薜荔和假地豆的粗提物及溶剂萃取部位对MT - 4人白血病癌细胞系的细胞毒性活性。
通过甲醇完全萃取制备节节花、薜荔和假地豆干燥叶的粗提物，使用极性递增的溶剂对粗提物进行连续萃取得到萃取部位。使用白血病癌细胞系（MT - 4）测定72小时暴露于提取物后与细胞存活对应的剂量反应。使用MTT[3 -（4,5 - 二甲基噻唑 - 2 - 基）- 2,5 - 二苯基溴化四氮唑]法在570nm处读取吸光度来评估细胞活力。与对照进行比较，每个样品孔中的细胞存活情况根据样品与对照的吸光度比值来确定。
节节花、薜荔和假地豆的粗提物均显示出细胞毒性，IC50值分别为89μg/ml、131μg/ml和81μg/ml。节节花和假地豆粗提物的石油醚和氯仿部位显示出较强的细胞毒性活性，但薜荔的氯仿和丁醇部位细胞毒性活性最高，IC50值分别为23μg/ml和26μg/ml。
节节花、薜荔和假地豆的粗提物对白血病细胞系MT - 4具有细胞毒性，并测定了IC50值。通过溶剂 - 溶剂萃取对粗提物进行分馏能够确定活性部位及其IC50值。我们认为细胞毒性活性可能归因于先前从这些植物中分离出的抗氧化化合物。
