基因操作和烟碱型乙酰胆碱受体生物学的最新进展。
Recent advances in gene manipulation and nicotinic acetylcholine receptor biology.
机构信息
Institute for Behavioral Genetics, University of Colorado at Boulder, UCB 447, Boulder, CO 80309, United States.
出版信息
Biochem Pharmacol. 2011 Oct 15;82(8):808-19. doi: 10.1016/j.bcp.2011.06.014. Epub 2011 Jun 16.
Abstract
Pharmacological and immunological methods have been valuable for both identifying some native nicotinic acetylcholine receptor (nAChR) subtypes that exist in vivo and determining the neurobiological and behavioral role of certain nAChR subtypes. However, these approaches suffer from shortage of subtype specific ligands and reliable immunological reagents. Consequently, genetic approaches have been developed to complement earlier approaches to identify native nAChR subtypes and to assess the contribution of nAChRs to brain function and behavior. In this review we describe how assembly partners, knock-in mice and targeted lentiviral re-expression of genes have been utilized to improve our understanding of nAChR neurobiology. In addition, we summarize emerging genetic tools in nAChR research.
摘要
药理学和免疫学方法对于鉴定体内存在的某些天然烟碱型乙酰胆碱受体（nAChR）亚型以及确定某些 nAChR 亚型的神经生物学和行为作用都非常有价值。然而，这些方法存在亚型特异性配体和可靠免疫试剂的短缺。因此，已经开发了遗传方法来补充早期的方法，以鉴定天然 nAChR 亚型，并评估 nAChR 对大脑功能和行为的贡献。在这篇综述中，我们描述了如何利用组装伙伴、基因敲入小鼠和靶向慢病毒的重新表达来提高我们对 nAChR 神经生物学的理解。此外，我们还总结了 nAChR 研究中的新兴遗传工具。
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