在子痫前期的发生发展中，基因型与凝血酶激活的纤溶抑制物（TAFI）的血浆水平之间的关系。

Association between genotype and plasma levels of thrombin-activated fibrinolysis inhibitor (TAFI) in the development of preeclampsia.

机构信息

Posgrado en Ciencias Químicobiológicas, Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México DF

出版信息

Thromb Res. 2011 Oct;128(4):e39-42. doi: 10.1016/j.thromres.2011.05.019. Epub 2011 Jun 25.

DOI:10.1016/j.thromres.2011.05.019

PMID:21708401

Abstract

INTRODUCTION

The objective was to evaluate if thrombin-activated fibrinolysis inhibitor (TAFI) polymorphisms (G505A, C1040T, and G-438A), and TAFIa plasma levels are associated with preeclampsia.

MATERIALS AND METHODS

In a case-control study design, we evaluated preeclampsia patients and women with uncomplicated pregnancies. The TAFI polymorphisms were determined by real-time PCR method, and TAFIa plasma levels were established with a chromogenic assay.

RESULTS

We included 87 women in each group. The TAFIa levels in the preeclampsia group were 20.4 μg/mL (CI 95% 17.3-23.5), while in the control group, they were significantly lower: 13.3 μg/mL (12.0-14.5, p 0.003). There were no differences in the genotype distribution or allelic frequency of TAFI polymorphisms between the two groups. In preeclampsia patients and controls heterozygous for the G505A polymorphism, the TAFIa values were 22.8 (16.7-28.9 μg/mL) and 13.2 (11.3-15.0 μg/mL, p 0.019), respectively. In G505A homozygous polymorphism the TAFIa values were 25.7 (18.7-32.6 μg/mL) and 13.5 (1.6-21.9 μg/mL, p 0.041), respectively. In the C1040T and G-438A TAFI wild type polymorphisms, the TAFIa values were 18.3 (12.5-23.9 μg/mL) and 11.5 (9.9-35.0, p 0.033), and 19.4 (10.9-27.9 μg/mL) and 12.5 (10.8-14.2 μg/mL, p 0.006), respectively, without differences in other genotypes.

CONCLUSIONS

Preeclampsia by itself may be responsible for the increase in TAFIa values rather than the presence of polymorphisms.

摘要

简介

目的是评估凝血酶激活的纤溶抑制物（TAFI）多态性（G505A、C1040T 和 G-438A）和 TAFIa 血浆水平与子痫前期的关系。

材料和方法

采用病例对照研究设计，我们评估了子痫前期患者和无并发症妊娠的妇女。通过实时 PCR 方法确定 TAFI 多态性，通过显色测定法确定 TAFIa 血浆水平。

结果

我们在每组中纳入了 87 名女性。子痫前期组的 TAFIa 水平为 20.4 μg/mL（95%CI 17.3-23.5），而对照组显著较低：13.3 μg/mL（12.0-14.5，p<0.003）。两组间 TAFI 多态性的基因型分布或等位基因频率无差异。在子痫前期患者和对照组杂合子 G505A 多态性中，TAFIa 值分别为 22.8（16.7-28.9 μg/mL）和 13.2（11.3-15.0 μg/mL，p=0.019）。在 G505A 纯合子多态性中，TAFIa 值分别为 25.7（18.7-32.6 μg/mL）和 13.5（1.6-21.9 μg/mL，p=0.041）。在 C1040T 和 G-438A TAFI 野生型多态性中，TAFIa 值分别为 18.3（12.5-23.9 μg/mL）和 11.5（9.9-35.0，p=0.033）和 19.4（10.9-27.9 μg/mL）和 12.5（10.8-14.2 μg/mL，p<0.0001），其他基因型无差异。