Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
Lett Appl Microbiol. 2011 Sep;53(3):379-82. doi: 10.1111/j.1472-765X.2011.03111.x. Epub 2011 Jul 18.
To simplify the electrotransformation process of Clostridium acetobutylicum, which currently needs to be performed in an anaerobic chamber, thus laborious and time-consuming.
The CAC2634 gene encoding PerR is a known peroxide regulon repressor in Cl. acetobutylicum. CAC2634 in a previously constructed Restriction-Modification system deficient Cl. acetobutylicum mutant SMB009 was disrupted using ClosTron method. The resulted mutant SMB012 can be electrotransformed in air with an efficiency of 1.2-3.1 × 10(3) transformants μg(-1) DNA.
We demonstrated that the disruption of CAC2634 in Cl. acetobutylicum enables its electrotransformation in air.
The electrotransformation process of Cl. acetobutylicum could be significantly simplified, especially when operating multiple electrotransformations.
简化目前需要在厌氧室内进行的丙酮丁醇梭菌（Clostridium acetobutylicum）电转化过程，该过程既费力又耗时。
编码 PerR 的 CAC2634 基因是 Cl. acetobutylicum 中已知的过氧化物调控子抑制剂。使用 ClosTron 方法敲除先前构建的限制-修饰系统缺陷型 Cl. acetobutylicum 突变体 SMB009 中的 CAC2634。得到的突变体 SMB012 可以在空气中进行电转化，转化效率为 1.2-3.1×10(3)转化体μg(-1)DNA。
我们证明了 Cl. acetobutylicum 中 CAC2634 的缺失使其能够在空气中进行电转化。
Cl. acetobutylicum 的电转化过程可以得到显著简化，特别是在进行多次电转化时。
Zotero 插件