血视网膜屏障处紧密连接蛋白表达的定量原位分析

Quantitative in situ analysis of claudin expression at the blood-retinal barrier.

作者信息

Xu Heping, Liversidge Janet

机构信息

Centre for Vision and Vascular Science, Queen's University Belfast, Belfast, UK.

出版信息

Methods Mol Biol. 2011;762:321-31. doi: 10.1007/978-1-61779-185-7_23.

DOI:10.1007/978-1-61779-185-7_23

PMID:21717367

Abstract

It is apparent that claudins are involved in signalling to and from cellular tight junctions (TJs) and control cell behaviour such as proliferation, differentiation, and migration. Methods to identify and measure specific claudins in TJs would, therefore, be useful to monitor TJ structure and functional integrity under physiological and pathological conditions. The molecular pathways involved in claudin signalling are not understood and are likely to become a focus for intensive research as better understanding of tight junction structure and function may provide opportunities for better drug delivery and absorption. In this chapter, we describe our method for quantitative analysis of specific claudins in TJ during the breakdown of the blood-retinal barrier in a mouse model of inflammatory uveitis, experimental autoimmune uveoretinitis (EAU).

摘要

显然，闭合蛋白参与细胞紧密连接（TJ）的信号传导，控制细胞行为，如增殖、分化和迁移。因此，识别和测量TJ中特定闭合蛋白的方法，对于监测生理和病理条件下TJ的结构和功能完整性将是有用的。闭合蛋白信号传导所涉及的分子途径尚不清楚，随着对紧密连接结构和功能的更好理解可能为更好的药物递送和吸收提供机会，这可能会成为深入研究的焦点。在本章中，我们描述了在炎症性葡萄膜炎实验性自身免疫性葡萄膜视网膜炎（EAU）小鼠模型中，在血视网膜屏障破坏过程中对TJ中特定闭合蛋白进行定量分析的方法。