Saar M, Kamradt J, Jung V, Stöckle M, Unteregger G
Klinik für Urologie und Kinderurologie , Universitätsklinikum des Saarlandes, Kirrberger Straße 1, 66421 Homburg/Saar, Deutschland.
Urologe A. 2011 Aug;50(8):961-7. doi: 10.1007/s00120-011-2630-7.
The clinical course of prostate cancer, the most common cancer in men, is very variable. Despite intense research activities over the years and besides histopathological criteria, prognostic markers that reliably predict tumor behavior and the necessity for treatment are still missing. A likely explanation for this fact is the lack of good tumor models, mimicking the in vivo situation. These models are not only essential for a better understanding of the pathogenesis of prostate cancer but also play an important role in the development of new therapeutic strategies. Since results of permanent cell culture experiments reflect only in part real tumor behavior and primary cultures from patient material cannot be grown indefinitely, novel approaches need to be developed to achieve reliable and clinically relevant prostate cancer research.In this work the development of several approaches for culturing primary prostate cancer tissue is illustrated and a forecast of future research plans utilizing xenograft models in mice is made.
前列腺癌是男性最常见的癌症，其临床病程差异很大。尽管多年来进行了大量研究活动，除了组织病理学标准外，可靠预测肿瘤行为和治疗必要性的预后标志物仍然缺失。这一事实的一个可能解释是缺乏能够模拟体内情况的良好肿瘤模型。这些模型不仅对于更好地理解前列腺癌的发病机制至关重要，而且在新治疗策略的开发中也发挥着重要作用。由于永久细胞培养实验的结果仅部分反映真实的肿瘤行为，并且来自患者材料的原代培养物不能无限期生长，因此需要开发新的方法来实现可靠且与临床相关的前列腺癌研究。在这项工作中，阐述了几种培养原发性前列腺癌组织方法的开发，并对利用小鼠异种移植模型的未来研究计划进行了预测。
