Pathology & Laboratory Medicine, NorthShore University Health System, 2650 Ridge Avenue, Evanston, IL 60201, USA.
J Clin Microbiol. 2011 Sep;49(9):3338-9. doi: 10.1128/JCM.00268-11. Epub 2011 Jul 6.
A real-time PCR assay was developed targeting the bla(KPC) responsible for Klebsiella pneumoniae carbapenemase (KPC)-mediated carbapenem resistance and was validated for testing colonies or enrichment broth cultures. The assay accurately detects KPC-containing strains with high analytical specificity and sensitivity.
建立了一种针对bla(KPC)的实时 PCR 检测方法,该基因负责导致肺炎克雷伯菌碳青霉烯酶(KPC)介导的碳青霉烯类耐药,并对其用于检测平板培养菌落或增菌肉汤培养物进行了验证。该方法对含 KPC 菌株具有高分析特异性和灵敏度。