Meier E, Kunz G, Haller O, Arnheiter H
Laboratory of Viral and Molecular Pathogenesis, National Institute of Neurological Disorders and Stroke, Bethesda, Maryland 20892.
J Virol. 1990 Dec;64(12):6263-9. doi: 10.1128/JVI.64.12.6263-6269.1990.
Upon stimulation with alpha/beta interferon, rat cells synthesize three Mx proteins. Sequence analysis of corresponding cDNAs reveals that these three proteins are derived from three distinct genes. One of the rat cDNAs is termed Mx1 because it is most closely related to the mouse Mx1 cDNA and because it codes for a nuclear protein that, like the mouse Mx1 protein, inhibits influenza virus growth. However, this protein differs from mouse Mx1 protein, in that it also inhibits vesicular stomatitis virus (VSV), a rhabdovirus. A second rat cDNA is more closely related to the mouse Mx2 cDNA and directs the synthesis of a cytoplasmic protein that inhibits VSV but not influenza virus. The third rat cDNA codes for a cytoplasmic protein that differs from the second one in only eight positions and has no detectable activity against either virus. These results indicate that rat Mx proteins have antiviral specificities not anticipated from the analysis of the murine Mx1 protein.
在用α/β干扰素刺激后，大鼠细胞合成三种Mx蛋白。对相应cDNA的序列分析表明，这三种蛋白源自三个不同的基因。其中一个大鼠cDNA被命名为Mx1，因为它与小鼠Mx1 cDNA关系最为密切，并且它编码一种核蛋白，该蛋白与小鼠Mx1蛋白一样，能抑制流感病毒的生长。然而，这种蛋白与小鼠Mx1蛋白不同，因为它还能抑制水泡性口炎病毒（VSV），一种弹状病毒。第二个大鼠cDNA与小鼠Mx2 cDNA关系更为密切，并指导合成一种细胞质蛋白，该蛋白能抑制VSV但不能抑制流感病毒。第三个大鼠cDNA编码一种细胞质蛋白，该蛋白与第二个蛋白仅在八个位置上不同，并且对两种病毒均无可检测到的活性。这些结果表明，大鼠Mx蛋白具有从对小鼠Mx1蛋白的分析中未预料到 的抗病毒特异性。
