Department of Biochemistry, Nencki Institute of Experimental Biology, 3 Pasteur St., 02-093 Warsaw, Poland.
Biochem Biophys Res Commun. 2011 Jul 29;411(2):235-40. doi: 10.1016/j.bbrc.2011.06.098. Epub 2011 Jun 26.
PMCA1-4 isoforms have been recently recognised as regulators of various signalling pathways in mammalian cells. PMCAs were found to interact with calcineurin A in an isoform specific manner. In this study we focus on the interaction of calcineurin A with PMCA4 and its effect on catecholamine secretion in PC12 cells with reduced PMCA2 or PMCA3 content. Reduction of synthesis of PMCA2 or PMCA3 led to upregulation of PMCA4 manifested by preferential interaction of PMCA4 with calcineurin A. On the other hand, we observed a significant reduction of dopamine secretion, which did not correspond with an increased Ca(2+). This result indicates that the interaction of PMCA4 with calcineurin A plays a regulatory role in the signalling during catecholamine secretion.
PMCA1-4 同种型最近被认为是哺乳动物细胞中各种信号通路的调节剂。已经发现 PMCAs 以同种型特异性的方式与钙调神经磷酸酶 A 相互作用。在这项研究中，我们专注于钙调神经磷酸酶 A 与 PMCA4 的相互作用及其对 PC12 细胞中儿茶酚胺分泌的影响，这些细胞中 PMCA2 或 PMCA3 的含量减少。PMCA2 或 PMCA3 的合成减少导致 PMCA4 的上调，表现为 PMCA4 与钙调神经磷酸酶 A 的优先相互作用。另一方面，我们观察到多巴胺分泌显著减少，但 Ca(2+) 没有增加。这一结果表明，PMCA4 与钙调神经磷酸酶 A 的相互作用在儿茶酚胺分泌的信号转导中起调节作用。
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