Centre for Molecular Biotechnology, Department of Chemical Engineering, Technical University of Catalonia, Barcelona, Spain.
J Neuroimmunol. 2011 Aug 15;237(1-2):13-22. doi: 10.1016/j.jneuroim.2011.05.010. Epub 2011 Jul 13.
Muscarinic acetylcholine receptors expression and signaling in the human Jurkat T cell line were investigated. Semiquantitative real-time PCR and radioligand binding studies, using a wide set of antagonist compounds, showed the co-existence of M(3), M(4), and M(5) subtypes. Stimulation of these subpopulations caused a concentration and time- dependent activation of second messengers and ERK signaling pathways, with a major contribution of the M(3) subtype in a G(q/11)-mediated response. In addition, we found that T-cell stimulation leads to increased expression of M(3) and M(5) both at transcriptional and protein levels in a PLC/PKCθ dependent manner. Our data clarifies the functional role of AChR subtypes in Jurkat cells and pave the way to future studies on the potential cross-talk among these subpopulations and their regulation of T lymphocytes immune function.
研究了人 Jurkat T 细胞系中毒蕈碱乙酰胆碱受体的表达和信号转导。使用广泛的拮抗剂化合物进行半定量实时 PCR 和放射性配体结合研究表明，M(3)、M(4)和 M(5)亚型共存。这些亚群的刺激导致第二信使和 ERK 信号通路的浓度和时间依赖性激活，其中 M(3)亚型在 G(q/11)介导的反应中起主要作用。此外，我们发现 T 细胞刺激以 PLC/PKCθ 依赖的方式导致 M(3)和 M(5)在转录和蛋白水平上的表达增加。我们的数据阐明了 AChR 亚型在 Jurkat 细胞中的功能作用，并为未来研究这些亚群之间的潜在串扰及其对 T 淋巴细胞免疫功能的调节铺平了道路。
