Institute of Physical Chemistry, Friedrich-Schiller University of Jena, 07743 Jena, Germany.
J Dermatol Sci. 2011 Sep;63(3):191-8. doi: 10.1016/j.jdermsci.2011.05.002. Epub 2011 Jun 23.
To visualise and characterise skin architecture, the tissue usually has to be destroyed and labelled.
The use of Fourier transform infrared (FTIR) spectroscopy as a label-free, minimally sample destructive method to define hair follicular structure has been explored and demonstrated in this paper.
Human scalp skin cryosections were imaged using FTIR microscopy and the data was subsequently analysed with N-FINDR spectral unmixing algorithm.
This resulted in an excellent distinction of known hair follicle tissue layers, which could be discerned based on their molecular structure.
The development of a minimally sample-destructive, label-free spectroscopy based technique that can differentiate layers of cells in the dermal papilla and connective tissue sheath in the mesenchyme of the hair follicle paves the way forward to identifying spectral markers important in wound healing and stem cell therapies.
为了可视化和描述皮肤结构，通常必须破坏和标记组织。
本文探讨了傅里叶变换红外（FTIR）光谱作为一种无标记、最小样本破坏性的方法，用于定义毛囊结构，并证明了其可行性。
使用 FTIR 显微镜对人头皮皮肤冷冻切片进行成像，随后使用 N-FINDR 光谱解混算法对数据进行分析。
这导致了对已知毛囊组织层的极好区分，这些组织层可以根据其分子结构来辨别。
开发一种最小样本破坏性、无标记的光谱技术，可以区分毛囊真皮乳头中的细胞层和间充质中的结缔组织鞘，为鉴定在创伤愈合和干细胞治疗中重要的光谱标记物铺平了道路。
