State Key Laboratory of Biocontrol, MOE Key Laboratory of Aquatic Product Safety and Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
Micron. 2011 Dec;42(8):884-91. doi: 10.1016/j.micron.2011.06.006. Epub 2011 Jun 24.
Siniperca chuatsi, Siniperca kneri, and Siniperca scherzeri are three of the most economically important sinipercid species. The ultrastructure and morphology of the mature spermatozoa of them are examined using transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The sperm consists of an acrosome-less head, a short midpiece and a long flagellum. Ultrastructurally, it has a homogeneously electron-dense nucleus in a granular pattern with nuclear lucent and a nuclear fossa excluding the centriolar complex. One to four mitochondria with lamellar cristae encircle the basal body of the flagellum in the midpiece. The cytoplasm surrounding the centrioles and the cylindric cytoplasmic channel contains glycogen granules and vesicles. Comprising the conventional 9+2 axoneme, vesicles and lateral fins, the sperm flagellum is inserted laterally on the nucleus, therefore the spermatozoon is asymmetrical. All of the spermatozoa of the three species are of the primitive or ect-aquasperm form and conform to the teleostean type II spermatozoa instead of the previously supposed type I. Variations in the shape of the heads, angles between the two centrioles, location of the cytoplasmic vesicles, mitochondrial number and structural characteristics of the lateral fins are notable among the three species. S. chuatsi is a sister-group of the other two species and is the most differentiated. The present study provides fresh insights to the comparative spermatology of Siniperca fishes and will be useful to the existing knowledge of the sinipercid fishes in systematic characters, biodiversity conservation and reproduction.
翘嘴红鲌、蒙古红鲌和青梢红鲌是三种最重要的鲌属经济鱼类。本文采用透射电镜和扫描电镜技术对其成熟精子的超微结构和形态进行了研究。精子由无头、短中段和长鞭毛组成。超微结构上，核呈颗粒状均匀电子致密，核透明，无中心粒复合体。中段有一个到四个带有板层嵴的线粒体环绕着鞭毛的基体。围绕中心粒和圆柱状细胞质通道的细胞质含有糖原颗粒和小泡。鞭毛由常规的 9+2 轴丝、小泡和侧鳍组成，侧向插入核上，因此精子是不对称的。三种鱼的精子都属于原始或外水生型，符合硬骨鱼类 II 型精子，而不是之前认为的 I 型。头部形状、两个中心粒之间的角度、细胞质小泡的位置、线粒体数量和侧鳍的结构特征在这三个物种中存在显著差异。翘嘴红鲌与其他两个物种是姐妹群，分化程度最高。本研究为鲌属鱼类比较精子学提供了新的见解，对鲌属鱼类在系统学特征、生物多样性保护和繁殖方面的现有知识将是有用的。
