Cadron Isabelle, Van Gorp Toon, Moerman Philippe, Waelkens Etienne, Vergote Ignace
Division of Gynecological Oncology, Department of Obstetrics and Gynecology, University Hospitals Leuven, Katholieke Universiteit Leuven, Leuven, Belgium.
Methods Mol Biol. 2011;755:155-63. doi: 10.1007/978-1-61779-163-5_12.
The evolution of ovarian cancer will highly depend on platinum sensitivity or resistance. In an attempt to better understand this mechanism of resistance, we combined laser microdissection (LMD) of ovarian tumor cells with mass spectrometric techniques. To obtain disease-specific markers we isolated ovarian tumor cells with LMD without contamination of surrounding cells. Proteins were extracted by chemical lysis and subsequent peptide and protein information was gathered using surface-enhanced laser desorption/ionization-time of flight mass spectrometry.
卵巢癌的进展在很大程度上取决于对铂的敏感性或耐药性。为了更好地理解这种耐药机制，我们将卵巢肿瘤细胞的激光显微切割（LMD）与质谱技术相结合。为了获得疾病特异性标志物，我们通过LMD分离卵巢肿瘤细胞，而不被周围细胞污染。通过化学裂解提取蛋白质，随后使用表面增强激光解吸/电离飞行时间质谱收集肽和蛋白质信息。
