Clothier K A, Leavens T, Griffith R W, Wetzlich S E, Baynes R E, Riviere J E, Tell L A
Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
J Vet Pharmacol Ther. 2012 Apr;35(2):113-20. doi: 10.1111/j.1365-2885.2011.01300.x. Epub 2011 Jul 18.
Tulathromycin is a macrolide antimicrobial labeled for treatment of bacterial pneumonia in cattle and swine. The purpose of the present research was to evaluate tissue concentrations of tulathromycin in the caprine species. A tandem mass spectrometry regulatory analytical method that detects the common fragment of tulathromycin in cattle and swine was validated with goat tissues. The method was used to study tulathromycin depletion in goat tissues (liver, kidney, muscle, fat, injection site, and lung) over time. In two different studies, six juvenile and 25 market-age goats received a single injection of 2.5 mg/kg of tulathromycin subcutaneously; in a third study, 18 juvenile goats were treated with 2.5, 7.5, or 12.5 mg/kg tulathromycin weekly with three subcutaneous injections. Mean tulathromycin tissue concentrations were highest at injection site samples in all studies and all doses. Lung tissue concentrations were greatest at day 5 in market-age goats while in the multi-dose animals concentrations demonstrated dose-dependent increases. Concentrations were below limit of quantification in injection site and lung by day 18 and in liver, kidney, muscle, and fat at all time points. This study demonstrated that tissue levels in goats are very similar to those seen in swine and cattle.
图拉霉素是一种大环内酯类抗菌药物，被批准用于治疗牛和猪的细菌性肺炎。本研究的目的是评估图拉霉素在山羊体内的组织浓度。一种检测牛和猪中图拉霉素共同片段的串联质谱监管分析方法在山羊组织中得到验证。该方法用于研究图拉霉素在山羊组织（肝脏、肾脏、肌肉、脂肪、注射部位和肺）中的随时间的消除情况。在两项不同的研究中，六只幼年山羊和25只成年山羊皮下注射了2.5mg/kg的图拉霉素；在第三项研究中，18只幼年山羊每周皮下注射2.5、7.5或12.5mg/kg的图拉霉素，共注射三次。在所有研究和所有剂量下，注射部位样本中图拉霉素的平均组织浓度最高。成年山羊肺组织浓度在第5天最高，而在多剂量动物中，浓度呈剂量依赖性增加。到第18天，注射部位和肺中的浓度低于定量限，在所有时间点肝脏、肾脏、肌肉和脂肪中的浓度也低于定量限。本研究表明，山羊体内的组织水平与猪和牛的非常相似。
