Frontier Lifeline, Chennai 600101, India.
Thromb Res. 2011 Oct;128(4):e49-53. doi: 10.1016/j.thromres.2011.05.026. Epub 2011 Jul 16.
Thrombospondin 1 and 2 are multidomain calcium-binding extracellular glycoproteins and they play a role in platelet aggregation, inflammatory response and assembly of connective tissue extracellular matrix. The association of thrombospondins (TSP) in the pathogenesis of coronary artery disease (CAD) and myocardial infarction (MI) is well established. The association of the TSP-1 (Asn700Ser, 2210A → G, rs2228262) and TSP-2 un-translated region (UTR) (3949T → G, rs8089) gene variations among South Indian CAD and MI patients has been examined in the present study.
We analyzed the thrombospondin polymorphisms in unrelated CAD patients (n = 511) and a subgroup with an event of MI (n = 173) compared with controls (n = 522). The polymorphisms were assessed using polymerase chain reaction, restriction fragment length analysis and the circulating TSP concentration were measured using enzyme linked immune-sorbent assay.
The prevalence of TSP-1 and TSP-2 alleles did not show any significant difference statistically, when compared controls against CAD/MI patients. The rare GG genotype of the N700S polymorphism was not observed among the studied population. Further, multiple regression analysis revealed that there was no significant risk for CAD (OR = 1.68; 95% CI 0.927 - 3.055; p = 0.087) or MI (OR = 1.84; 95% CI 0.846 - 4.007; p = 0.124) for the GA genotype. The GA genotype showed no impact on clinical characteristics of the CAD patients and their circulating TSP-1 levels. A similar non-association was observed for the TSP-2 in 3949T → G polymorphism (GG genotype) for CAD (OR = 0.64; 95% CI 0.278 - 1.455; p = 0.636) and MI (OR = 0.53; 95% CI 0.166 - 1.675; p = 0.278).
Our data suggests that the presence of thrombospondin-1 (rs2228262) and thrombospondin-2 (rs8089) variants need not be considered a risk for coronary artery disease or myocardial infarction among South Indians.
血小板反应蛋白 1 和 2 是具有多个结构域的钙结合细胞外糖蛋白，在血小板聚集、炎症反应和结缔组织细胞外基质组装中发挥作用。血小板反应蛋白（TSP）在冠状动脉疾病（CAD）和心肌梗死（MI）发病机制中的关联已得到充分证实。本研究检测了南印度 CAD 和 MI 患者中 TSP-1（Asn700Ser，2210A→G，rs2228262）和 TSP-2 非翻译区（UTR）（3949T→G，rs8089）基因变异的关联。
我们分析了 511 例无关 CAD 患者（病例组）和 173 例发生 MI 的亚组（病例组）与 522 例对照组之间的血小板反应蛋白多态性。使用聚合酶链反应、限制性片段长度分析评估多态性，并使用酶联免疫吸附试验测量循环 TSP 浓度。
与 CAD/MI 患者相比，TSP-1 和 TSP-2 等位基因的流行率在统计学上无显著差异。在所研究的人群中未观察到 N700S 多态性的罕见 GG 基因型。此外，多元回归分析显示，GA 基因型对 CAD（OR=1.68；95%CI 0.927-3.055；p=0.087）或 MI（OR=1.84；95%CI 0.846-4.007；p=0.124）无显著风险。GA 基因型对 CAD 患者的临床特征及其循环 TSP-1 水平没有影响。在 3949T→G 多态性（GG 基因型）中也观察到 TSP-2 的非关联，对 CAD（OR=0.64；95%CI 0.278-1.455；p=0.636）和 MI（OR=0.53；95%CI 0.166-1.675；p=0.278）也没有影响。
我们的数据表明，血小板反应蛋白-1（rs2228262）和血小板反应蛋白-2（rs8089）变异的存在在南印度人中不必被认为是冠状动脉疾病或心肌梗死的危险因素。
