测定屋尘中杂色曲霉菌过敏原。

Quantification of the Aspergillus versicolor allergen in house dust.

机构信息

Ottawa-Carleton Institute of Chemistry, Carleton University, Ottawa, ON K1S5B6, Canada.

出版信息

J Immunol Methods. 2011 Sep 30;372(1-2):89-94. doi: 10.1016/j.jim.2011.06.034. Epub 2011 Jul 13.

DOI:10.1016/j.jim.2011.06.034

Abstract

Aspergillus versicolor, a fungus commonly found on damp building materials, produces the allergen, Asp v 13. Here we report a sensitive Asp v 13 capture ELISA for A. versicolor spores and spore- and mycelial fragments in house dust samples. The method is based on a double polyclonal capture ELSIA. The detection limits for Asp v 13 antigen and A. versicolor spores without dust were 2.44 pg and 12 ng (ca. 110 spores). Detection limits for Asp v 13 and A. versicolor spores in sieved house dust samples were 1.0 ng and 7.8 μg per gram dry weight house dust, respectively. This detection limit is lower than for other house dust allergen immunoassays including for Stachybotrys chartarum, Aspergillus fumigatus, but much lower than that from Alternaria alternata.

摘要

杂色曲霉，一种常见于潮湿建筑材料上的真菌，产生过敏原，Asp v 13。在这里，我们报告了一种用于房屋灰尘样本中杂色曲霉孢子和孢子及菌丝片段的敏感 Asp v 13 捕获 ELISA。该方法基于双多克隆捕获 ELISA。Asp v 13 抗原和不含灰尘的杂色曲霉孢子的检测限分别为 2.44 pg 和 12 ng（约 110 个孢子）。在筛选的房屋灰尘样本中，Asp v 13 和杂色曲霉孢子的检测限分别为每克干燥灰尘 1.0 ng 和 7.8 μg。该检测限低于其他房屋灰尘过敏原免疫测定，包括烟曲霉、构巢曲霉，但远低于链格孢。