Department of Analytical Chemistry, Faculty of Chemistry, Razi University, Kermanshah, Iran.
Anal Chim Acta. 2011 Sep 2;701(1):1-5. doi: 10.1016/j.aca.2011.05.046. Epub 2011 Jun 2.
Headspace solid phase microextraction using anodized aluminum fiber in combination with capillary GC-MS was utilized as monitoring technique for the collection and detection of the volatile compounds of Echinophora platyloba DC. Experimental parameters, including the sample weight, extraction temperature, extraction time and humidity effect, desorption time and desorption temperature were examined and optimized. Using HS-SPME followed by GC-MS, 53 compounds were separated and identified in E. platyloba DC, which mainly included E-β ocimene (47.63%), R-D-decalactone (13.28%), α-pinene (7.43%) and nonane (6.71%). Compared with hydrodistillation (HD), HS-SPME, provides the advantages of a small amount of sample, timesaving, simplicity and cheapness. To the best of our knowledge, this is the first report on using anodized aluminum fiber in solid-phase microextraction coupled to headspace for the investigation of volatile fraction of medicinal plant.
采用阳极氧化铝纤维的顶空固相微萃取与毛细管 GC-MS 联用作为监测技术，用于收集和检测苍耳的挥发性化合物。考察并优化了实验参数，包括样品量、萃取温度、萃取时间和湿度效应、解吸时间和解吸温度。采用 HS-SPME 结合 GC-MS，从苍耳中分离和鉴定出 53 种化合物，主要包括 E-β 罗勒烯（47.63%）、R-D-癸内酯（13.28%）、α-蒎烯（7.43%）和壬烷（6.71%）。与水蒸馏（HD）相比，HS-SPME 具有样品用量少、省时、简单、廉价等优点。据我们所知，这是首次报道采用阳极氧化铝纤维在固相微萃取中结合顶空用于研究药用植物的挥发性成分。
