Sykes J E, Lopes-Cardozo M
Laboratory of Veterinary Biochemistry, State University of Utrecht, The Netherlands.
Glia. 1990;3(6):495-501. doi: 10.1002/glia.440030608.
Glial cells were isolated from the cerebra of 7-day-old rats and maintained in culture in a chemically defined medium that favours the development of oligodendrocytes. Acetate, butyrate, or albumin-bound hexanoate, octanoate, decanoate, laurate, myristate, palmitate, oleate, linoleate, or arachidonate was added to the culture medium. The incorporation of [3H]acetate into fatty acids and cholesterol and [35S]sulphate into sulphatide, and the activities of the oligodendrocyte marker enzymes 2',3'-cyclic-nucleotide 3'-phosphodiesterase and glycerol 3-phosphate dehydrogenase were measured. The composition of the glial cell population (the number of astrocytes and oligodendrocytes) in these cultures was studied by immunocytochemistry. Results show that 1) long-chain fatty acids depress the synthesis of fatty acids, cholesterol, and sulphatide; and 2) the presence of long-chain, in contrast to short-chain, fatty acids in the culture medium lowers the activities of 2',3'-cyclic-nucleotide 3'-phosphodiesterase and glycerol 3-phosphate dehydrogenase and decreases the number of oligodendrocytes. Our results suggest that long-chain fatty acids exert a negative influence on the development of oligodendrocytes in the culture system used.
从7日龄大鼠的大脑中分离出神经胶质细胞，并在有利于少突胶质细胞发育的化学限定培养基中进行培养。向培养基中添加乙酸盐、丁酸盐或与白蛋白结合的己酸盐、辛酸盐、癸酸盐、月桂酸盐、肉豆蔻酸盐、棕榈酸盐、油酸盐、亚油酸盐或花生四烯酸盐。测定了[3H]乙酸盐掺入脂肪酸和胆固醇以及[35S]硫酸盐掺入硫脂的情况，以及少突胶质细胞标记酶2',3'-环核苷酸3'-磷酸二酯酶和甘油3-磷酸脱氢酶的活性。通过免疫细胞化学研究了这些培养物中神经胶质细胞群体的组成（星形胶质细胞和少突胶质细胞的数量）。结果表明：1）长链脂肪酸抑制脂肪酸、胆固醇和硫脂的合成；2）与短链脂肪酸相比，培养基中长链脂肪酸的存在会降低2',3'-环核苷酸3'-磷酸二酯酶和甘油3-磷酸脱氢酶的活性，并减少少突胶质细胞的数量。我们的结果表明，长链脂肪酸对所用培养系统中少突胶质细胞的发育产生负面影响。
