Department of Medicine (RMH), University of Melbourne, Victoria, Australia.
J Magn Reson Imaging. 2011 Oct;34(4):774-84. doi: 10.1002/jmri.22669. Epub 2011 Jul 18.
To examine the long-term consequences of manganese exposure due to the use of manganese-enhanced magnetic resonance imaging (MEMRI) in a model of closed head injury, the fluid-percussion injury (FPI) model.
Two groups of adult male Wistar rats (n = 72) were studied with either MEMRI, whereby rats receive MnCl(2) (100 mg/kg intraperitoneally) 24 hours prior to scanning, or standard MRI (sMRI) with no contrast agent. Rats from both groups underwent FPI or sham injury and were longitudinally assessed for 6 months for neurological toxicity using behavioral tests, EEG recording, and MRI scanning.
Regardless of whether they received FPI, MEMRI animals showed progressive signs of cerebral toxicity compared with sMRI rats, including significantly reduced weight gain, progressive brain volume decrease, and increased anxiety and depressive-like behaviors.
Long-term structural and functional consequences of using manganese as a contrast agent for MRI can confound experimental outcomes and must be taken into account when designing longitudinal imaging studies using manganese-enhanced MRI.
在闭合性颅脑损伤模型（液压冲击伤模型）中，使用锰增强磁共振成像（MEMRI）检查锰暴露的长期后果。
本研究纳入了两组成年雄性 Wistar 大鼠（n=72），一组进行 MEMRI 检查，即在扫描前 24 小时腹腔内给予 MnCl2（100mg/kg）；另一组进行标准 MRI（sMRI）检查，不使用对比剂。两组大鼠均接受液压冲击伤或假手术处理，并在 6 个月内进行神经毒性的纵向评估，使用行为测试、脑电图记录和 MRI 扫描。
无论是否接受液压冲击伤，进行 MEMRI 的动物与接受 sMRI 的动物相比，均显示出进行性的脑毒性迹象，包括体重增长明显减少、脑体积逐渐缩小，以及焦虑和抑郁样行为增加。
使用锰作为 MRI 对比剂的长期结构和功能后果可能会干扰实验结果，因此在使用锰增强 MRI 进行纵向成像研究时，必须加以考虑。
