D1Gazi University Faculty of Medicine, Department of Microbiology and Clinical Microbiology, 06500, Besevler, Ankara, Turkey.
Asian Pac J Trop Med. 2011 Jan;4(1):16-9. doi: 10.1016/S1995-7645(11)60024-2. Epub 2011 Jan 15.
To investigate which cytokines are produced after acute infection of mice with Toxoplasma gondii (T. Gondii) RH strain.
Mus domesticus domesticus mice in infected group were inoculated with with highly virulent T. Gondii RH strain by intraperitoneally. Serum samples were obtained from infected and non-infected mice for cytokine levels for ELISA assay.
The concentrations of tumor necrosis factor-α, interferon-γ, interleukin (IL)-10 and IL-12 in the cardiac blood sample of the infected mice were significantly higher than those in uninfected controls (P<0.05). The levels of transforming growth factor-1β decreased in mice infected with T. gondii compared to those of the controls, the decrease was statistically significant (P<0.05). No significant difference was observed in levels of IL-4 between infected and healty control groups (P>0.05).
According to our findings, immune response into T helper type 1 was predominant during acute T. gondii infection. Further characterization and purification of Toxoplasma molecule(s) implicated in the regulation of cytokines could lead to the development of new drug prospects to control Toxoplasma infection.
研究刚地弓形虫 RH 株急性感染后产生的细胞因子。
用高毒力的刚地弓形虫 RH 株经腹腔接种感染组小家鼠。采集感染和未感染小家鼠的血清样本，用于 ELISA 法检测细胞因子水平。
感染鼠心肌血样中肿瘤坏死因子-α、干扰素-γ、白细胞介素（IL）-10 和 IL-12 的浓度明显高于未感染对照组（P<0.05）。与对照组相比，感染弓形虫的小鼠转化生长因子-β水平下降，差异有统计学意义（P<0.05）。感染组与健康对照组之间的白细胞介素-4 水平无显著差异（P>0.05）。
根据我们的发现，在刚地弓形虫急性感染期间，免疫反应以辅助性 T 细胞 1 型为主。对参与细胞因子调节的弓形虫分子的进一步鉴定和纯化可能会开发出控制弓形虫感染的新药物前景。
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