Laboratoire de Génie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires, Institut de Chimie et Biochimie Moléculaire et Supramoléculaire, Université Lyon1, CNRS 5246 ICBMS, Bat. CPE, 43 Bd du 11 Nov., 69622 Villeurbanne, France.
Lab Chip. 2011 Sep 7;11(17):3006-10. doi: 10.1039/c1lc20246d. Epub 2011 Jul 20.
We are reporting here a new technology for the straightforward production of integrated microarrays. The approach is based on the use of adhesive supports enabling (i) the immobilization of biomolecules as microarrays (up to 2500 spots per cm(2)) and (ii) the easy assembly of these microarrays with complex 3D structures such as 96-well bottomless microplates or polymer and glass microfluidic networks. The analytical performances of the system were demonstrated for sandwich protein detection (C-reactive protein) and hybridization assays, both in classical 96-well microplate format and microfluidic environment.
我们在此报告一种用于直接制备集成微阵列的新技术。该方法基于使用粘附性支持物,使(i)生物分子作为微阵列固定化(每平方厘米可达 2500 个斑点)和(ii)这些微阵列与复杂的 3D 结构(如 96 孔无底微板或聚合物和玻璃微流控网络)的易于组装。该系统的分析性能通过夹心蛋白检测(C-反应蛋白)和杂交测定来证明,这两种方法都在经典的 96 孔微板格式和微流控环境中进行。